WTIP ELISA Kit (Wilms Tumor Protein 1-Interacting Protein)

INTENDED USE

Human WTIP ELISA kit can measure concentrations of Wilms tumor protein 1-interacting protein (WTIP, WT1-interacting protein) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 46.875 pg/ml.

INTRODUCTION

WTIP, also known as Wilms Tumor Protein 1 Interacting Protein is a gene that codes for a protein involved in processes within cells. It has been linked to conditions, like Chromosome 19Q13.11 Deletion Syndrome and Prolidase Deficiency. Scientific studies have revealed that WTIP plays a role in hypertrophy and interacts with noncanonical Wnt signaling. Furthermore research has identified a variant in WTIP (p.Y233F). Provided evidence of its involvement in complex diseases particularly hypertrophic cardiomyopathy (HCM).

In terms of its function WTIP acts as a regulator under conditions by facilitating the association between prolyl hydroxylases and VHL proteins which leads to the efficient breakdown of HIF1A (hypoxia inducible factor 1 alpha). This suggests that WTIP may play a role in responses, to low oxygen levels and hypoxia.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-WTIP antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this WTIP ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 78.13, 156.25, 312.5, 625, 1250, 2500, 5000 pg/ml.
Reactivity: Human
Sensitivity: 46.875 pg/ml
Range: 78.125 – 5000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for WTIP, no cross reactivity or interference between WTIP and analogues was detected.
– Recovery: Serum (85 – 102%), EDTA Plasma (86 – 101%), Heparin Plasma (85 – 101%).
– Linearity: Serum (88 – 101%), EDTA Plasma (88 – 100%), Heparin Plasma (84 – 95%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Role of Asxl1 in kidney podocyte development via its interaction with Wtip. Biochem Biophys Res Commun. (2015) 466 (3): 560-6. Moon S., et al.
2. Wnt Signaling Interactor WTIP (Wilms Tumor Interacting Protein) Underlies Novel Mechanism for Cardiac Hypertrophy. Circ Genom Precis Med. (2022) 15 (4): e003563. De Jong H.N., et al.
3. WT1-interacting protein (Wtip) regulates podocyte phenotype by cell-cell and cell-matrix contact reorganization. Am J Physiol Renal Physiol. (2012) 302 (1): F103-15. Kim J.H., et al.

ADDITIONAL INFORMATION

• Full Name: WTIP ELISA Kit (Wilms Tumor Protein 1-Interacting Protein)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 46.875 pg/ml

OTHER RELATED ELISA KITS

• Peptide YY ELISA Kit
• Copeptin ELISA Kit
• PICP ELISA Kit

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