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Peptide YY ELISA Kit (PYY)

Full Name: Peptide YY ELISA Kit (PYY)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 18.75 pg/ml

INTRODUCTION

Peptide YY (PYY) is a 36-amino acid peptide hormone released by L cells of the distal gastrointestinal tract in response to feeding. Along with neuropeptide Y and pancreatic polypeptide, it comprises the NPY family of biologically active peptides that regulate various physiological processes. Best known as a key regulator of appetite and food intake, PYY signaling also influences gastric motility, water and electrolyte absorption, and bone metabolism.

Circulating at low fasting levels, nutrient ingestion dramatically stimulates PYY release into the bloodstream in proportion to caloric load. This activates Y2 receptors on hypothalamic neurons, suppressing the activity of orexigenic neurons coexpressing neuropeptide Y and agouti-related peptide (AgRP). Consequently, PYY elicits anorexigenic effects. Rodent studies revealed intraperitoneal PYY injection decreases food intake and body weight gain. Conversely Y2 receptor deletion causes hyperphasia and obesity, consistent with the critical role of PYY as a satiety signal. Beyond energy balance, PYY may also modulate bone remodeling based on observations of increased bone formation markers in Y2 receptor knockout mice.

However, direct translation of PYY’s metabolic benefits to humans has encountered challenges. Short-term infusion indeed decreased appetite and food intake, but chronically elevated PYY levels induced by pharmaceutical intervention failed to consistently show weight lowering effects. Nonetheless, bariatric surgery upregulating endogenous PYY production has proven effective against obesity. This highlights the importance of physiological PYY activity for energy homeostasis. Efforts continue to develop improved Y2R and PYY-based therapies that potently suppress appetite without undesirable side effects.

INTENDED USE

Human peptide YY ELISA kit can measure concentrations of PYY (peptide YY, peptide tyrosine tyrosine, PYY-I) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-PYY antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this peptide YY ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.5, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PYY, no cross reactivity or interference between PYY and analogues was detected.
– Recovery: Serum (87 – 104%), EDTA Plasma (87 – 100%), Heparin Plasma (85 – 102%).
– Linearity: Serum (93 – 105%), EDTA Plasma (91 – 100%), Heparin Plasma (82 – 100%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. The Role of Neuropeptide Y and Peptide YY in the Development of Obesity via Gut-brain Axis. Curr Protein Pept Sci. (2019) 20 (7): 750-758. Wu Y., et al.
  2. PYY, a Therapeutic Option for Type 2 Diabetes? Clin Med Insights Endocrinol Diabetes. (2020) 13: 1179551419892985. Guida C. and Ramracheya R.
  3. Peptide YY: a neuroendocrine neighbor of note. Peptides. (2007) 28 (2): 345-51. Cox H.M.
  4. PYY is a negative regulator of bone mass and strength. Bone. (2019) 127: 427-435. Leitch V.D., et al.

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