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Copeptin ELISA Kit (CPP)

Full Name: Copeptin ELISA Kit (CPP)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 18.75 ng/ml

INTRODUCTION

Copeptin refers to the C-terminal segment of the arginine vasopressin (AVP) prohormone remaining after enzymatic cleavage releases the active hormone mediating water homeostasis. As precursor and product exhibit equimolar release into circulation, quantifying stable copeptin serves as a surrogate biomarker closely mirroring AVP levels. Thereby copeptin measurements enable indirect assessment of the difficult-to-measure vasopressin hormone regulating fluid balance and vascular tone in health and disease states like diabetes insipidus, heart and kidney dysfunction.

The precursor pre/pro-vasopressin encodes both the active hormone arginine vasopressin (AVP) as well as copeptin. After directed cleavage eventsExc, copeptin and AVP are secreted at equimolar levels, however copeptin exhibits far greater stability in circulation with levels reflecting endogenous AVP tone. By binding specific receptors on kidney tubules, vascular smooth muscle and platelets, the peptide hormone AVP exhibits anti-diuretic properties while also vasoconstricting blood vessels to increase pressure. This makes it a difficult analyte – both short-lived yet tightly regulated to maintain osmotic balance.

In contrast, copeptin mirrors AVP concentrations proportional to osmotic status and hemodynamic stress because processing co-synthesizes the two. Consequently, the longer-lived peptide copeptin serves as an alternative biomarker accurately reflecting circulating AVP levels useful for assessing hydration status and diagnosing disorders of vasopressin dysregulation in much simpler immunoassays. Beyond mirroring AVP dynamics, cardiovascular and renal disease states actually perturb copeptin metabolism directly, enhancing its predictive value as a risk stratification biomarker integrating underlying pathologies like heart failure, diabetes and metabolic syndrome.

INTENDED USE

Human copeptin ELISA kit can measure concentrations of CPP (copeptin, CT-proAVP, C-terminal provasopressin, vasopressin-neurophysin 2-copeptin) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-CPP antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this copeptin ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 50, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 ng/ml (1.56 – 100 pmol/L)
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CPP, no cross reactivity or interference between CPP and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (85 – 103%), Heparin Plasma (85 – 104%).
– Linearity: Serum (85 – 103%), EDTA Plasma (86 – 101%), Heparin Plasma (89 – 94%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. Investigation of copeptin levels in foetal congenital central nervous system anomalies. Obstet Gynaecol. (2021) 41 (1): 49-54. Çilgin H.J
  2. Utility of copeptin and standard inflammatory markers in the diagnostics of upper and lower urinary tract infections. BMC Urol. (2015) 15: 67. Masajtis-Zagajewska A., et al.
  3. Renal function changes in patients with subclinical hyperthyroidism: a novel postulated mechanism. Endocrine. (2023) 82 (1): 78-86. Allam M.M., et al.
  4. Serum copeptin level predicts a rapid decrease of overhydration after kidney transplantation. Clin Chem Lab Med. (2014) 52 (9): 1297-303. Kolonko A., et al.

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