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PICP ELISA Kit

Full Name: PICP ELISA Kit (C-Terminal Propeptide of Collagen Alpha-1(I) Chain)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 37.5 pg/ml

INTRODUCTION

PICP, or C-terminal propeptide of procollagen type I, refers to a peptide fragment cleaved during the extracellular processing of procollagen type I into mature collagen type I. As a stoichiometric byproduct generated alongside collagen, PICP levels in serum reflect the biosynthesis of type I collagen, the predominant structural protein in bone and soft connective tissue. Consequently, immunoassays detecting circulating PICP concentrations provide a direct biomarker of collagen turnover, offering utility in research and for monitoring anabolic disease states.

During collagen assembly, C- and N-terminal propeptides flank the procollagen chains, enabling proper triple helical folding prior to extracellular cleavage by specific proteinases. This liberates mature collagen for fibril formation as well as propeptides. Since PICP peptide is secreted into circulation on a 1:1 molar ratio with collagen, its levels correlate with collagen synthesis rates. As bone tissue represents the primary source of type I collagen and turnover marker, serum PICP levels closely associate with osteoblastic bone formation activity.

Correspondingly, PICP proves useful to gauge pathological shifts in bone turnover characteristic of primary hyperparathyroidism, Paget’s disease and osteoporosis. Furthermore, serum PICP shows clinical utility for monitoring responses to anti-resorptive or anabolic therapy, often rising prior to mineralization markers. Quantitative enzyme-linked immunosorbent PICP assays also facilitate estimation of heart failure severity and liver fibrosis stage, reflecting remodeling changes in soft connective tissue.

INTENDED USE

Human PICP ELISA kit can measure concentrations of C-terminal propeptide of collagen alpha-1(I) chain (PICP, C-propeptide type I procollagen) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-PICP antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this PICP ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Human
Sensitivity: 37.5 pg/ml
Range: 62.5 – 4000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PICP, no cross reactivity or interference between PICP and analogues was detected.
– Recovery: Serum (86 – 104%), EDTA Plasma (85 – 99%), Heparin Plasma (87 – 96%).
– Linearity: Serum (89 – 105%), EDTA Plasma (82 – 94%), Heparin Plasma (85 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. Procollagen type I carboxy-terminal propeptide (PICP) and MMP-2 are potential biomarkers of myocardial fibrosis in patients with hypertrophic cardiomyopathy. Cardiovasc Pathol. (2019) 43: 107150. Yang C., et al.
  2. PICP as bone formation and NTx as bone resorption marker in patients with chronic renal failure. Eur J Med Res. 1998 Feb 21;3(1-2):81-8. Franke S., et al.
  3. Collagen: scaffold for repair or execution. Cardiovasc Res. (1997) 33 (3): 535-9. Jensen L.T. and Høst N.B.
  4. Serum PICP as a bone formation marker in 89Sr and external beam radiotherapy of prostatic bony metastases. Br J Radiol. (1997) 70 (834): 594-8. Papatheofanis FJ.

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