Vitamin D3 Receptor ELISA Kit (VDR)

Full Name: Vitamin D3 Receptor ELISA Kit (VDR)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.375 ng/ml

INTRODUCTION

The receptor, for vitamin D3, known as VDR is a type of receptor for carrying out the genetic effects of active vitamin D called 1,25 dihydroxyvitamin D3 (calcitriol). Its main function is to act as a transcription factor that responds to molecules (ligands) by forming a complex with another receptor called X receptor when bound to calcitriol. This complex then binds to regions within the DNA of target genes known as vitamin response elements. As a result it triggers changes in gene expression by recruiting molecules involved in gene transcription. VDR activation directly or indirectly influences hundreds of genes related to cell growth control, differentiation processes, metabolism regulation and immune system functions.

Among the genes affected by vitamin D3 receptor activity are those involved in promoting calcium absorption like TRPV6 and calbindin. Additionally VDR increases the production of RANKL which helps in the formation of osteoclasts for bone resorption and releasing calcium from bones. At the time it stimulates the production of osteocalcin and osteopontin which aid in bone formation by supporting osteoblasts activities. Through these mechanisms VDR signaling ensures calcium levels within the body while maintaining bone health. Moreover it also plays a role in modulating both adaptive immunity responses well as exerting anti proliferative effects on certain cells. It even has an influence on processes, like hair growth cycles and expression of peptides.

INTENDED USE

Human vitamin D3 receptor ELISA kit can measure concentrations of signal transducer and activator of VDR (vitamin D3 receptor, nuclear receptor subfamily 1 group I member, 25-dihydroxyvitamin D3 receptor, vitamin D receptor, vitamin D1) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-VDR antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this vitamin D3 receptor ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.625, 1.25, 2.5, 5, 10, 20, 40 ng/ml.
Reactivity: Human
Sensitivity: 0.375 ng/ml
Range: 0.625 – 40 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for VDR, no cross reactivity or interference between VDR and analogues was detected.
– Recovery: Serum (87 – 102%), EDTA Plasma (86 – 101%), Heparin Plasma (82 – 100%).
– Linearity: Serum (85 – 104%), EDTA Plasma (84 – 101%), Heparin Plasma (80 – 94%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects. Physiol Rev. (2016) 96 (1): 365-408. Christakos S., et al.
2. Vitamin D3 action within the ovary – an updated review. Physiol Res. (2020) 69 (3): 371-378. Grzesiak M.
3. Vitamin D receptor (VDR)-mediated actions of 1α,25(OH)₂vitamin D₃: genomic and non-genomic mechanisms. Best Pract Res Clin Endocrinol Metab. (2011) 25 (4): 543-59. Haussler M.R., et al.
4. Transcriptional targets of the vitamin D3 receptor-mediating cell cycle arrest and differentiation. J Nutr. (1999) 129 (2S Suppl): 581S-586S. Freedman L.P.

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• STAT3 ELISA Kit
• Annexin A3 ELISA Kit