Superoxide Dismutase ELISA Kit (SOD)

Full Name: Superoxide Dismutase ELISA Kit (SOD)
Reactivity: Human
Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
Sensitivity: 0.469 ng/ml

INTRODUCTION

Superoxide dismutase (SOD) aids in the breakdown of superoxide molecules into oxygen and hydrogen peroxide. It is an antioxidant enzyme, as it works to defend cells against the harm caused by reactive oxygen particles. There are two forms of SOD: copper-zinc SOD, which is copper and zinc cofactor bound to each other and cytochrome c, and copper-only SOD (CuZn-SOD) that has only one single molecule of copper without a zinc cofactor. The gene coding for CuZn-SOD is located on chromosome 11 at position 15.2. The protein is 481 amino acids long containing approx 45% amino acid sequence identity with the protein coded for by the P301L mutation in CCS that causes Leigh syndrome. Mutations in the gene encoding CuZn-SOD lead to Leigh syndrome.

Superoxide dismutase enzymes are found in all living organisms and there are three different types; (a). those present in the cytoplasm (Cu/Zn-SOD), (b). those in the mitochondria (Mn-SOD) and (c). those in the mitochondria and nuclei (Fe-SOD). These enzymes catalyse hydrogen peroxide dismutation by a non-enzymatic method. They have a copper (Cu) element that, when combined with oxygen, creates hydroxyl radicals. These radicals then combine with hydrogen peroxide to create water and two molecules of diatomic oxygen. A zinc component of the Cu/Zn-SOD enzyme additionally combines with hydrogen peroxide to create hydroxyl radicals and superoxide, which subsequently react with a sulphide molecule to create hydrogen sulphide and sulphur dioxide. Cells use the SOD enzyme in their mitochondria to remove the superoxide generated naturally inside cells by oxidation reactions, such as those that occur in respiration.

INTENDED USE

Human superoxide dismutase ELISA kit can be used for analysing concentrations of superoxide dismutase (SOD, cytosolic, IPOA, Cu-Zn SOD) in human serum, plasma, biological fluids and tissue homogenates.

CONTENT

All reagents supplied with need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with SOD
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

The following superoxide dismutase ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.781, 1.562, 3.125, 6.25, 12.5, 25, 50 ng/ml.
Reactivity: Human
Sensitivity: 0.469 ng/ml
Range: 0.781 – 50 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for SOD, no cross reactivity or interference between SOD and analogues was detected.
– Recovery: Serum (86 – 101%), EDTA Plasma (90 – 100%), Heparin Plasma (89 – 105%).
– Linearity: Serum (85 – 104%), EDTA Plasma (85 – 103%), Heparin Plasma (85 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Inhibition of copper-zinc superoxide-dismutase activity by selected environmental xenobiotics. Environ Toxicol Pharmacol. (2018) 58: 105-113. Lewandowski Ł., et al.
2. Superoxide dismutase: pharmacological developments and applications. Adv Pharmacol. (1992) 23: 109-61. Omar B.A., et al.
3. The role of manganese superoxide_dismutase in health and disease. J Inherit Metab Dis. 1998 Aug;21(5):598-603. Robinson B.H.

OTHER RELATED ELISA KITS

• Sclerostin ELISA Kit
• TNFSF11/RANKL ELISA Kit
• Immunoglobulin A ELISA Kit