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Sclerostin ELISA Kit (SOST)

Full Name: Sclerostin ELISA Kit (SOST)
Reactivity: Human
Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
Sensitivity: 37.5 pg/ml

INTRODUCTION

Sclerostin (SOST) is a protein that is produced by osteocytes, these cells are mainly found in bone tissues. It is a family member of the inhibitory secreted frizzled-related proteins (SFRPs). It is a single polypeptide chain with a molecular weight of 25 kDa. SOST contains a cysteine-rich region that is predominately responsible for its inhibitory activity. towards the Wnt signalling pathway. Sclerostin is also produced in bone and other tissue cells by bone morphogenetic proteins (BMPs) including; BMP-2, -4, -5 and -7. This is a result of both genetic expression and mRNA translation.

One of the main functions of sclerostin is to act as an inhibitor to bone formation and it is able carry this out role by binding and inhibiting the activity of LRP5. Upon binding to LRP5, it is able to inhibit the activation of the Wnt signalling pathway, this will leads to affecting the downstream processes of bone formation and remodelling. The net affect will be a reduction in the formation of bone. Other important functions of SOST include regulation skeletal homeostasis and apoptosis. It is found to influence mineralization processes for example calcium deposition, phosphate deposition and soft tissue remodelling. The main function of sclerostin is the negative control of bone formation and remodelling process. It plays an important role in regulating the activation of Wnt signalling pathway.

INTENDED USE

Human sclerostin ELISA kit can be used for detecting concentrations of SOST (sclerostin) in human serum, plasma, biological fluids and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with SOST
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

The following sclerostin ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml.
Reactivity: Human
Sensitivity: 37.5 pg/ml
Range: 62.5 – 400 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for SOST, no cross reactivity or interference between SOST and analogues was detected.
– Recovery: Serum (88 – 101%), EDTA Plasma (87 – 103%), Heparin Plasma (94 – 98%).
– Linearity: Serum (87 – 101%), EDTA Plasma (85 – 93%), Heparin Plasma (84 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Role and mechanism of action of sclerostin in bone. (2017) 96: 29-37. Delgado-Calle J., et al.
  2. Anti-Sclerostin Antibodies in Osteoporosis and Other Bone Diseases. J Clin Med. 2020 Oct 26;9(11):3439. Fabre S., et al.
  3. Sclerostin: From Molecule to Clinical Biomarker. Int J Mol Sci. (2022) 23 (9): 4751.

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