Rat MMP-2 ELISA Kit (Matrix Metalloproteinase 2)

Full Name: Rat MMP-2 ELISA Kit (Matrix Metalloproteinase 2)
Reactivity: Rat
Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
Sensitivity: 0.188 ng/ml

INTRODUCTION

Matrix metalloproteinase 2 (MMP-2) is regarded as a house keeping gene since it represents high constitutive activity. In rats, it has been found that MMP-2 protein and mRNA levels are greatly increase within 24 hours post-MI and the reach a peak around the 14 days point. Comparatively to age-matched controls, heart transplant patients have considerably lower levels of MMP-2. MMPs participate in cell migration, proliferation and death as well as the degradation of extracellular matrix components. This is why these proteases are often involved with diseases such as cancer or infection.

A common feature of MMPs is that they can be regulated by other genes through either transcriptional or posttranscriptional events. Some examples of transcriptional regulation include p53 which upregulates its expression during cell cycle arrest and growth factor signalling via nuclear factor κB. MMPs can be found in many different cellular compartments. They participate in cell migration, proliferation, and death as well as the degradation of extracellular matrix components. Typically, they are localized to an intracellular compartment that is close to a source of MMP activity such as a cell surface protease or receptor.

Other functions of MMP-2 include the regulation of blood vessel formation, during the process of tissue regeneration/repair and in mediating the tissue breakdown that is essential for tumor invasion. It is also a vital mediator during the atherosclerotic plaque rupture process. MMP-2 also aids in tumor invasion, metastasis and angiogenesis. Tumor cells can force MMP-2 out of the cell surfaces, thereby causing them to invade the surrounding tissue. In addition, MMPs facilitate the recruitment of blood vessels into tumors as a means for angiogenesis; such mechanisms help stabilize these tumors against apoptosis. In addition, expression of MMP-2 is increased when inflammatory mediators are released.

INTENDED USE

Rat MMP-2 ELISA kit is designed for detecting amounts of matrix metalloproteinase 2 (MMP-2, gelatinase A, 72kDa gelatinase, CLG4 and collagenase type IV-A) using rat samples of tissue homogenates, biological fluids, serum or plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

1. One 96-Well Plate: Pre-coated with anti-MMP-2 antibody.
2. Standards: Lyophilized recombinant.
3. Sample/Standard Dilution Buffer.
4. Biotinylated-labelled Antibody.
5. Antibody Dilution Buffer.
6. HRP-Streptavidin Conjugate (SABC).
7. SABC Dilution Buffer.
8. TMB Substrate.
9. Wash Buffer (25x).
10. Plate Sealer.
11. Product Instructions.

TYPICAL RESULTS

For this rat MMP-2 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5.0, 10.0, 20.0 ng/ml.
Reactivity: Rat
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for MMP-2, no cross reactivity or interference between MMP-2 and analogues was detected.
– Recovery: Serum (89 – 103%), EDTA Plasma (91 – 99%), Heparin Plasma (90 – 102%).
– Linearity: Serum (88 – 103%), EDTA Plasma (86 – 99%), Heparin Plasma (82 – 91%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. MMP-2 regulates rat ventral prostate development in vitro. Dev Dyn. (2010) 239 (3): 737-46. Bruni-Cardoso A., et al.
2. Effects of ovariectomy and resistance training on MMP-2 activity in rat calcaneal tendon. Connect Tissue Res. (2010) 51 (6): 459-66. Pereira GB, et al.
3. Canstatin stimulates migration of rat cardiac fibroblasts via secretion of matrix metalloproteinase-2. Am J Physiol Cell Physiol. (2017) 312 (3): C199-C208. Okada M., et al.

OTHER RELATED ELISA KITS

• MMP-13 ELISA Kit
• MMP-9 ELISA Kit
• MMP-3 ELISA Kit