MMP-3 ELISA Kit (Matrix Metalloproteinase-3)

INTENDED USE

Human MMP-3 ELISA kit is designed for determining in vitro quantitative amounts of matrix metalloproteinase-3 (MMP-3, MMP3, stromelysin or transin) in human plasma, serum, cell lysates, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 0.094 ng/ml.

BACKGROUND

Matrix metalloproteinase-3 (MMP3, MMP-3) is a proteoglycanase closely related to MMP1 and it displays many different substrate specificities. It has an estimated molecular mass of 54 kDa and the complete primary structure is made up of 477 residues which includes a signal peptide.

MMP-3 can be released as a secreted metalloprotease which is formed mainly by connective tissue cells. It can also synergistically degrade the extracellular matrix (major components) alongside other metalloproteases. It can activate other MMPs (MMP1, MMP7 and MMP9) during connective tissue remodelling, wound repair, tumor initiation and progression of atherosclerosis. There is also evidence that it can cause damage to the blood-spinal cord barrier (BSCB), this can be view as a functional equivalent of the blood-brain barrier following spinal cord injury (SCI). Other findings also implicate it in the in exacerbating the effects seen during traumatic brain injury (TBI) which is the result of causing disruption to the blood-brain barrier (BBB).

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with MMP-3.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of human matrix metalloproteinase-3 (MMP-3, MMP3, transin and stromelysin) using current MMP-3 ELISA kit was 0.094 ng/ml. The dynamic range for this assay is 0.156 – 10 ng/ml.

REFERENCES

1. Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. (2015) 53 (5): 585-600. Review. Craig V.J., et al.
2. Polymorphisms of MMP-1 and MMP-3 and susceptibility to rheumatoid arthritis. A meta-analysis. Z Rheumatol. (2015) 74 (3): 258-62. Review. Zhang C., et al.
3. Role of matrix metalloproteinase-3 in neurodegeneration. J Neurochem. (2011) 116 (1): 22-32. Review. Kim E.M. and Hwang O.
4. Regulation of MMP3 by laminin alpha 4 in human osteoarthritic cartilage. Scand J Rheumatol. (2011) 40 (6): 494-6. Fuerst F.C., et al.

ADDITIONAL INFORMATION

• Full Name: MMP-3 ELISA Kit (Matrix Metalloproteinase-3)
• Reactivity: Human
• Sample Type: Serum, Other Biological Fluids, Plasma, Cell Culture Supernatant, Cell Lysates
• Sensitivity: 0.094 ng/ml

OTHER RELATED ELISA KITS

• MMP-1 ELISA Kit
• MMP-7 ELISA Kit
• MMP-9 ELISA Kit