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Rabies Virus IgG ELISA Kit (Dog)

INTENDED USE

Dog rabies virus IgG ELISA kit is designed to analyse quantitative amounts of IgG antibodies against canine rabies virus (RV) in dog serum or plasma samples. This assay has a quantitative and qualitative standard range.

BACKGROUND

Rabies virus (RV) is known to infect mainly warm blooded species, there are two different forms of it. Paralytic rabies (usually spinal cord infections) and furious rabies (usually brain infections). The virus is found in saliva and this represent the most favoured form of transmission, sporadic cases through aerosol infections have also been discovered. The infection of dogs by rabies virus (RV) in most cases tends to be fatal, carnivores such as; domestic dogs, cats, rodents and bats are known to be responsible transmitting infections to dogs and humans. The following procedure relies of the use of a semi purified virus which is designed as a rapid screen in order to detect rabies antibodies in dog plasma and serum samples.

RABIES VIRUS IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips: Coated with purified rabies antigens
  • Strip Holder.
  • ELISA Buffer.
  • HRPO Conjugated anti-species antibodies.
  • Positive Control.
  • Negative Control.
  • Substrate A.
  • Substrate B.
  • Wash Solution (200x Conc.).
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of IgG antibodies to canine rabies virus (RV) using current dog rabies virus IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Canine Rabies Virus (RV) IgG: ELISA
– Positive control should be ≥ 1.250 OD units (450nm).
– Negative control should be lower than 0.500 OD units (450nm) and give an endpoint titer of ≤ 50.
– Qualitative (Positive – Negative): Sample is scored positive if the OD is higher than the OD of the negative control plus 0.100.
– Quantitative (End Point Titer): End-point titer of the sample is the dilution which gives an extinction just above the OD of the negative control plus 0.150.

REFERENCES

  1. Immunoglobulin response to rabies virus immunization in dogs. Vaccine. (1991) 9 (9): 611-2. Jayakumar R. and Ramadass P.
  2. Identification of rabies virus mimotopes screened from a phage display peptide library with purified dog anti-rabies virus serum IgG. Virus Res. (2013) 174 (1-2): 47-51. Yang L., et al.
  3. Standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera. J Biol Stand. (1989) 17 (4): 291-309. Thraenhart O. and Ramakrishnan K.
  4. Measurement of rabies-specific antibodies in carnivores by an enzyme-linked immunosorbent assay. J Wildl Dis. (1988) 24 (2): 246-58. Barton L.D. and Campbell J.B.

ADDITIONAL INFORMATION

  • Full Name: Rabies Virus IgG ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative, Quantitative

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