Rabies Virus IgG ELISA Kit (Dog)


Dog rabies virus IgG ELISA kit is designed to analyse quantitative amounts of IgG antibodies against canine rabies virus (RV) in dog serum or plasma samples. This assay has a quantitative and qualitative standard range.


Rabies virus (RV) is known to infect mainly warm blooded species, there are two different forms of it. Paralytic rabies (usually spinal cord infections) and furious rabies (usually brain infections). The virus is found in saliva and this represent the most favoured form of transmission, sporadic cases through aerosol infections have also been discovered. The infection of dogs by rabies virus (RV) in most cases tends to be fatal, carnivores such as; domestic dogs, cats, rodents and bats are known to be responsible transmitting infections to dogs and humans. The following procedure relies of the use of a semi purified virus which is designed as a rapid screen in order to detect rabies antibodies in dog plasma and serum samples.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips: Coated with purified rabies antigens
  • Strip Holder.
  • ELISA Buffer.
  • HRPO Conjugated anti-species antibodies.
  • Positive Control.
  • Negative Control.
  • Substrate A.
  • Substrate B.
  • Wash Solution (200x Conc.).
  • Stop Solution.
  • Plastic Cover Seal.


The standard range of IgG antibodies to canine rabies virus (RV) using current dog rabies virus IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.


– Canine Rabies Virus (RV) IgG: ELISA
– Positive control should be ≥ 1.250 OD units (450nm).
– Negative control should be lower than 0.500 OD units (450nm) and give an endpoint titer of ≤ 50.
– Qualitative (Positive – Negative): Sample is scored positive if the OD is higher than the OD of the negative control plus 0.100.
– Quantitative (End Point Titer): End-point titer of the sample is the dilution which gives an extinction just above the OD of the negative control plus 0.150.


  1. Immunoglobulin response to rabies virus immunization in dogs. Vaccine. (1991) 9 (9): 611-2. Jayakumar R. and Ramadass P.
  2. Identification of rabies virus mimotopes screened from a phage display peptide library with purified dog anti-rabies virus serum IgG. Virus Res. (2013) 174 (1-2): 47-51. Yang L., et al.
  3. Standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera. J Biol Stand. (1989) 17 (4): 291-309. Thraenhart O. and Ramakrishnan K.
  4. Measurement of rabies-specific antibodies in carnivores by an enzyme-linked immunosorbent assay. J Wildl Dis. (1988) 24 (2): 246-58. Barton L.D. and Campbell J.B.


  • Full Name: Rabies Virus IgG ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative, Quantitative



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford