Canine Rabies Virus IgG ELISA Kit (RV IgG)

Full Name: Caine Rabies Virus IgG ELISA Kit (RV IgG)
Reactivity: Dog
Sample Type: Serum, Plasma
Sensitivity: Qualitative, Quantitative

BACKGROUND

There are two different types of the rabies virus (RV); (a). paralytic, which typically affects the spinal cord and (b). furious, which mostly attacks the brain. Saliva is the main method through which this virus is transmitted. This virus is transmitted to humans and dogs by carnivores like bats, domestic dogs, cats and rodents. Warm-blooded animals are primarily affected by this virus. It is characterized by fever, headache, anxiety, and sometimes hydrophobia. The virus travels to the nervous system and spreads through the brain via synapses. This causes neurological damage, which includes paralysis of the muscles that control respiration. Dogs are often paralyzed in this area and will eventually die from asphyxiation if left untreated for a period of time.

The rabies virus is be passed from person to person or animal to animal by direct contact. The aerosolization of droplets is another means of transmission. An infection causes a sudden onset of fever, headaches, and fatigue. Confusion, disorientation and hallucinations may develop six to ten days after infection, eventually leading to convulsions and coma. Infected spinal cord will result in paralysis but not convulsions. An infection causes a sudden onset of fever, headaches, and fatigue. Infected spinal cord will result in paralysis but not convulsions.

This virus frequently results in mortality in canines. The following strategy is suitable for rapid screening procedures because it employs a semi-purified rabies virus, which is ideal for evaluating rabies antibodies that may be present in canine serum and plasma samples.

INTENDED USE

Dog rabies virus IgG ELISA kit is designed to analyse quantitative amounts of IgG antibodies against canine rabies virus (RV IgG) in canine/dog serum or plasma samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips: Coated with purified rabies antigens
Strip Holder.
ELISA Buffer.
HRPO Conjugated anti-species antibodies.
Positive Control.
Negative Control.
Substrate A.
Substrate B.
Wash Solution (200x Conc.).
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of IgG antibodies to canine rabies virus (RV) using current canine rabies virus IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Positive control should be ≥ 1.250 OD units (450nm).
– Negative control should be lower than 0.500 OD units (450nm) and give an endpoint titer of ≤ 50.
– Qualitative (Positive – Negative): Sample is scored positive if the OD is higher than the OD of the negative control plus 0.100.
– Quantitative (End Point Titer): End-point titer of the sample is the dilution which gives an extinction just above the OD of the negative control plus 0.150.

REFERENCES

Immunoglobulin response to rabies virus immunization in dogs. Vaccine. (1991) 9 (9): 611-2. Jayakumar R. and Ramadass P.
Identification of rabies virus mimotopes screened from a phage display peptide library with purified dog anti-rabies virus serum IgG. Virus Res. (2013) 174 (1-2): 47-51. Yang L., et al.
Standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera. J Biol Stand. (1989) 17 (4): 291-309. Thraenhart O. and Ramakrishnan K.
Measurement of rabies-specific antibodies in carnivores by an enzyme-linked immunosorbent assay. J Wildl Dis. (1988) 24 (2): 246-58. Barton L.D. and Campbell J.B.

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