Canine Rabies Virus IgM ELISA Kit (RV IgM)

Full Name: Canine Rabies Virus IgM ELISA Kit (RV IgM)
Reactivity: Dog
Sample Type: Serum, Plasma
Sensitivity: Qualitative, Quantitative

BACKGROUND

Rabies virus (RV) exists in two different forms; furious rabies which mostly infects the brain and paralytic rabies which most often infects the spinal cord. This virus predominately infects warm blooded species, saliva is the most common form of transmission and carnivores for example; bats, domestic dogs, cats and rodents are found to transmit this infection to humans and dogs. Therefore, it is not possible to determine where the virus was initially infected and therefore treatment is not focused on one area.

Rabies virus is transmitted through direct contact with infected animals or humans. Also, droplets in aerosolised form is another mode of transmission. Infection results in sudden onset of fever, headache and lethargy. This is followed by confusion, disorientation and hallucination that may progress to convulsions and coma within 6–10 days following infection. If the spinal cord has been infected then paralysis will occur rather than convulsions. There is no specific treatment for rabies.

In majority of cases, the infection of dogs by this virus can be fatal. The following protocol is suitable for carrying out rapid screening procedures, this is due to the use a semi purified rabies virus which is ideal for measuring rabies antibodies which may be present in serum and plasma samples of dogs.

INTENDED USE

Canine rabies virus IgM ELISA kit is intended for detecting quantitative quantity of IgM antibodies against canine rabies virus (RV IgM) in dog plasma and serum samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Strips: Coated with purified rabies antigens.
• Negative Control (Freeze Dried).
• Positive Control (Freeze Dried).
• HRPO Conjugated anti-species antibodies.
• ELISA Buffer.
• Substrate A.
• Substrate B.
• Wash Solution (200x Conc.).
• Stop Solution.
• Plastic Cover Seal.
• Strip Holder.

SENSITIVITY

The standard range of IgM antibodies to canine rabies virus (RV) using current canine rabies virus IgM ELISA Kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Quantitative (End Point Titer): End-point titre of the sample is the dilution which gives an extinction just above the OD of the negative control plus 0.150.
– Qualitative (Positive – Negative): Sample is scored positive if the OD is higher than the OD of the negative control plus 0,200.
– Positive control should be ≥ 0.700 OD units (450nm).
– Negative control should be lower than 0.350 OD units (450nm) and give an endpoint titer of ≤ 30.

REFERENCES

1. Anti-rabies virus IgM in serum and cerebrospinal fluid from rabid dogs. Southeast Asian J Trop Med Public Health. (1986) 17 (4): 550-7. Tingpalapong M., et al.
2. Reinvestigating the role of IgM in rabies virus postexposure vaccination. J Virol. (2013) 87 (16): 9217-22. Dorfmeier C.L., et al.
3. Mechanisms of rabies virus neutralization. Virology. (1993) 194 (1): 302-13. Flamand A., et al.
4. Immunoglobulin response to rabies virus immunization in dogs. Vaccine. (1991) 9 (9): 611-2. Jayakumar R. and Ramadass P.

OTHER RELATED ELISA KITS

• Rabies Virus IgG ELISA Kit (Dog)
• CCV (Canine Coronavirus) IgG ELISA Kit (Dog)
• CDV (Canine Distemper Virus) IgM ELISA Kit (Dog)