CCV IgM ELISA Kit (Canine Coronavirus)

Full Name: CCV IgM ELISA Kit (Canine Coronavirus)
Reactivity: Canine/Dog
Sample Type: Plasma, Serum
Sensitivity: Quantitative

BACKGROUND

Canine Coronavirus (CCV) causes intestinal disease in dogs and is a member of the coronaviridae family. In most cases the infected dogs experience a complete recover and develop immunity to CCV. The virus invades and replicates in the villi of the small intestine, in majority of instances this can also make the cells more susceptible to infection by parvoviruses.

Looking at clinical history, clinical sign and performing antigen detection are often carried out in the process of diagnosing the virus. Infected dogs can spread it to healthy ones through the fecal-oral route as well as by contact with contaminated objects or surfaces. There is no specific treatment for CCV in dogs. In addition to treating secondary infections, supportive treatment may also involve intravenous fluids and oxygen therapy.

Following procedure is designed to determine corona virus antibodies in blood or plasma samples, it is also well known that majority of positive dogs are potential virus carriers and therefore will shed CCV.

INTENDED USE

CCV IgM ELISA kit can be used for analysing IgM antibodies against canine coronavirus (CCV)(dog) in blood and plasma samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips Coated With CCV Proteins.
Weak Positive Control.
Negative Control.
Strip Holder.
HRPO-Conjugated (IgM)(Anti-Species) Antibody.
Wash Solution (200x Concentrated).
ELISA Buffer.
Substrate Buffer A.
Substrate Buffer B.
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of IgM antibodies to canine coronavirus (CCV) using current CCV IgM ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Qualitatively: Sample is scored positive if the OD is higher than 2.5 x OD of the negative control.
– Quantitatively (End Point Titer):
< 30: No antibodies found.
90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
> 810: High titer of antibodies found in recovered diseased animals.

REFERENCES

Isolation, tissue distribution and molecular characterization of two recombinant canine coronavirus strains. Vet Microbiol. (2011) 151 (3-4): 238-44. Ntafis V., et al.
Sequence analysis of divergent canine coronavirus strains present in a UK dog population. Virus Res. (2009) 141 (1): 21-5. Erles K. and Brownlie J.
Antibody testing against canine coronavirus by immunoperoxidase plaque staining. Vet Res Commun. (2001) 25 (4): 327-36. Soma T., et al.
Canine coronavirus highly pathogenic for dogs. Emerg Infect Dis. (2006) 12 (3): 492-4. Buonavoglia C., et al.

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