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CAV (Canine Adenovirus) ELISA Kit (Dog)

INTENDED USE

Canine adenovirus ELISA kit can be used for measuring antibodies against canine adenovirus (CAV)(dog) in plasma and serum samples. This assay has a quantitative and qualitative standard range.

BACKGROUND

Canine adenovirus (CAV) is a complex disease which is frequently found in either wild or even domestic dogs, usually in majority of cases the dogs that are infected tend to only have subclinical infections which mostly recover completely. At present there are two sub-groups that have been identified CAV-1 and CAV-2. This virus can be spread through the blood, urine, saliva, nasal discharge and faeces of infected dogs and in most cases is contracted through the nose or mouth and it replicated within the tonsils. The virus often infects the kidneys and the liver and as an incubation period between 4-7 days. Diagnosis of this disease can be made through a combination antibody detection, clinical history and clinical signs, also an elevated level of antibody titer to CAV-1 can be observed. The disease can be often confused with canine parvovirus, since both lead to low white blood cell count and bloody diarrhea in unvaccinated, young dogs.

CANINE ADENOVIRUS ELISA KIT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips Coated With Canine Adenovirus Proteins.
  • Strip Holder.
  • HRPO-Conjugated (Anti-Species) Antibody.
  • CAV Weak Positive Control.
  • CAV Negative Control.
  • Wash Solution (200x Concentrated).
  • ELISA Buffer.
  • Substrate Buffer A.
  • Substrate Buffer B.
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of antibodies directed to CAV using current canine adenovirus (CAV) ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Canine Adenovirus (CAV): ELISA
– Sample is scored positive if the OD is higher than 2.5 x OD of the negative control.
– End point titer:
≤ 30: No antibodies found.
90 – 270: Diseased (antibodies found, probably shedding CAV, retest in 2 months) or not diseased (low titer normally found incompletely recovered dogs but they still might be virus carriers).
≥ 810: High titer of antibodies found 8-10 weeks after infection or vaccination.

REFERENCES

  1. Construction and characterization of a recombinant canine adenovirus expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus. J Vet Med Sci. (2010) 72 (8): 1035-40. Cai J., et al.
  2. The serological and virological investigation of canine adenovirus infection on the dogs. Scientific World Journal. (2013) 2013: 587024. Bulut O., et al.
  3. Detection and differentiation of CAV-1 and CAV-2 by polymerase chain reaction. Vet Res Commun. (2001) 25 (1): 77-84. Hu R.L., et al.
  4. A real-time PCR assay for quantification of canine adenoviral vectors. J Virol Methods. (2010) 163 (1): 129-36. Segura M.M., et al.
  5. Characterization of the genome of a vaccine strain of canine adenovirus type 1. Virus Genes. (1988) 2 (1): 69-81. Liu Y.C., et al.

ADDITIONAL INFORMATION

  • Full Name: CAV (Canine Adenovirus) ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Serum, Plasma
  • Sensitivity:  Qualitative, Quantitative

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