PYY3 ELISA Kit (Putative Peptide YY-3)

Full Name: PYY3 ELISA Kit (Putative Peptide YY-3)
Reactivity: Human
Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
Sensitivity: 3.75 pg/ml

INTRODUCTION

Putative peptide YY-3 (PYY3) is described as a gut protein and it is released from the colon and ileum. The peptide fragment is secreted by intestinal L cells when expose to food that has crossed the blood-brain barrier. It binds to the Y2 receptor in the VMH, the activation of this receptor is known to decrease in the levels of NPY mRNA in neurons of the orexigenic arm. It is a signal of satiety and a regulator of food intake and energy expenditure. The presence in plasma predicts feeling full after eating and enhances glucose tolerance, however, there is evidence that it also increases food consumption by rodents by decreasing the activity of the anorexigenic neuropeptide neuropeptide Y (NPY) within the hypothalamus in response to ingestion. Putative peptide YY-3 levels decrease during fasting but increase after a meal.

PYY 3-36 injections have been proven to decrease food intake and appetite in research subjects who were obese. The concentration in the blood is inversely related to the amount of calories consumed from meals. The generation of a pro-inflammatory cytokine (TNF-), as well as the “turnover” of bacterial communities in the large intestine and intestinal inflammatory responses, are both inhibited by it. PYY3 is thought to influence pancreatic function by boosting the generation of insulin and activating beta cells.

Putative peptide YY-3 is a hormone that plays an essential part in the regulation of energy homeostasis by stimulating feelings of satiety and suppressing hunger. The concentration levels vary throughout the day and are highest in early morning hours before breakfast, reaching a peak when individuals consume their first meal. This finding suggests that a rise in PYY 3-36 may be correlated with having been seen eating, or simply thinking about eating during this time period.

INTENDED USE

Human PYY3 ELISA kit can detect amounts of putative peptide YY-3 (PYY3, PYY 3) present is human plasma, serum, biological fluids and tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-PYY3 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this PYY3 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 6.25, 12.5, 25, 50, 100, 200, 400 pg/ml.
Reactivity: Human
Sensitivity: 3.75 pg/ml
Range: 6.25 – 400 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PYY3, no cross reactivity or interference between PYY3 and analogues was detected.
– Recovery: Serum (90 – 101%), EDTA Plasma (87 – 105%), Heparin Plasma (85 – 104%).
– Linearity: Serum (96 – 105%), EDTA Plasma (85 – 95%), Heparin Plasma (85 – 91%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. PYY3-36: Beyond food intake. Front Neuroendocrinol. (2015) 38: 1-11. Stadlbauer U., et al.
2. Effects of PYY3-36 and GLP-1 on energy intake, energy expenditure, and appetite in overweight men. Am J Physiol Endocrinol Metab. (2014) 306 (11): E1248-56. Schmidt J.B., et al.
3. Peptides and obesity: the PYY3-36 story. Regul Pept. (2004) 119 (1-2): 1-2. Park A. and Bloom S.R.

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