CGRP ELISA Kit (Calcitonin Gene Related Peptide)

Full Name: CGRP ELISA Kit (Calcitonin Gene Related Peptide)
Reactivity: Human
Sample Type: Serum, Tissue Homogenates, Biological Fluids, Plasma
Sensitivity: 9.375 pg/ml

INTRODUCTION

A family of peptides, including calcitonin, calcitonin receptor stimulating peptide, adrenomedullin, adrenomedullin 2 (intermedin), and amylin, includes calcitonin gene-related peptide (CGRP). It is formed from thyroid C cells and it is stored and released into the nervous system. The human peptide can be either alpha-CGRP or beta-CGRP. The alpha peptide is 37 amino acids neuropeptide and this is produce from alternative splicing of calcitonin/CGRP gene. However, less is known about the beta-CGRP apart from the fact that it is located to a separate gene to the alpha and it differs by three amino acids. The presence of it and its receptors in the brain may be an indicator for certain neuropathic pain conditions including chronic neuropathic pain which is often referred to as reflex sympathetic dystrophy.

The central and peripheral neurons are responsible for producing CGRP. It performs as a vasodilating peptide and aids in the transmission of nociception. It is derived from motor neuron body cells that have been synthesised within the spinal cord ventral horn. It also found to display effects on the homeostasis of calcium. It functions as an appetite suppressant and aids the secretion of gastric acid. Other roles include; increasing heart rate, releasing of pituitary hormones and temperature homeostasis.

CGRP is not only released in the central and peripheral neurons, but it also exists at high levels in the hypothalamus. It has been discovered to have an impact on food consumption and weight. There are several “work functions” including; decreasing pain perception, increasing heart rate and blood pressure, increasing gastric acid secretion, releasing of pituitary hormones and temperature homeostasis. The hypothalamus region where hunger and satiety signals are produced contains the receptors. NPY is also found in this area, suggesting that it may be involved in regulating appetite and food intake.

INTENDED USE

Human CGRP ELISA kit can be used to measure amounts of calcitonin gene related peptide (CGRP) present is human serum, tissue homogenates, plasma and other biological fluids.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti-CGRP antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this CGRP ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.6225, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for CGRP, no cross reactivity or interference between CGRP and analogues was detected.
– Recovery: Serum (85 – 98%), EDTA Plasma (85 – 98%), Heparin Plasma (86 – 99%).
– Linearity: Serum (82 – 100%), EDTA Plasma (83 – 100%), Heparin Plasma (82 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

CGRP Monoclonal Antibodies for Migraine: Rationale and Progress. (2017) 31 (6): 487-501. Yuan H., et al.
Calcitonin gene-related peptide (CGRP): role in migraine pathophysiology and therapeutic targeting. Expert Opin Ther Targets. (2020) 24 (2): 91-100. Wattiez A.S., et al.
CGRP physiology, pharmacology, and therapeutic targets: migraine and beyond. Physiol Rev. (2023) 103 (2): 1565-1644. Russo A.F. and Hay D.L.
The big CGRP flood – sources, sinks and signalling sites in the trigeminovascular system. Headache Pain. (2018) 19 (1): 22. Messlinger K.

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