Pregnenolone ELISA Kit

INTENDED USE

Human pregnenolone ELISA kit is a reliable quantitative procedure for measuring pregnenolone (3β-hydroxypregn-5-en-20-one) in human serum. This assay has a minimum sensitivity detection limit of 0.05 ng/ml.

BACKGROUND

Pregnenolone (which is also referred to as 3β-hydroxypregn-5-en-20-one) is a one of the most common precursor the majority of adrenal and gonadal steroids. It was the first steroid hormone which was derived from cholesterol from the pathway of steroidogenesis. It is produced within the mitochondria as a result of the C-20 side chain of cholesterol being cleaved by the P-450SCC enzyme. After it has been produced, it can be utilised by two pathways of steroidogenesis. Studies of the concentrations in relation to sex and age differences has been found to indicate that maximum levels occur in men at 17 ages and in women at age 16. Whilst the minimum levels occur in men at age 28 and in women at age 37.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterwells: Break apart strips that are coated with anti-pregnenolone antibody (polyclonal).
• Calibrators 0-6: Concentrations 0, 0.1, 0.4, 1.6, 6.4, 25.6 ng/ml.
• Control: Contains anti pregnenolone in a human serum matrix.
• Pregnenolone Biotin Enzyme Conjugate (50x).
• Streptavidin-Horseradish Peroxide (HRP) Conjugate (50x).
• Biotin Conjugate Buffer.
• HRP Conjugate Buffer.
• Wash Buffer (10x Concentrated).
• TMB Substrate.
• Stopping Solution.

SENSITIVITY

The minimum sensitivity detection limit of 3β-hydroxypregn-5-en-20-one (pregnenolone) using current pregnenolone ELISA kit was approximately 0.05 ng/ml. The dynamic range for this assay is 0.1 – 25.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.054 ng/ml
– Specificity (Cross Reactivity): Pregnenolone (100%), Progesterone (6.0%), Dehydroisoandrosterone (5.2%), 5α-Androstandiol (4.7%), Epiandrosterone (1.0%), Pregnenolone Sulfate (0.4%), Androstandione (0.3%), 5α-Androsterone (0.3%), DHEAS (0.2%), Etiocholanolone (0.1%), Aldosterone, Androstenedione, Cholesterol, Corticosterone, 5α-DHT, 17β-Estradiol, Estriol, Testosterone (< 0.1%).
– Intra-Assay Variability: 8.7 – 9.8%
– Inter-Assay Variability: 8.4 – 11.7%
– Recovery: 101.5 – 118.3%
– Linearity: 82.9 – 102.2%
– Expected Normal Values: Males (0.38 – 3.5 ng/ml), Females (0.31 – 3.8 ng/ml).

REFERENCES

1. Neurosteroid regulation of central nervous system development. Pharmacol Ther. (2007) 116 (1): 107-24. Review. Mellon S.H.
2. Progesterone-synthesis by the human placenta. Placenta. (2005) 26 (4): 273-81. Review. Tuckey RC.
3. Role of pregnenolone, dehydroepiandrosterone and their sulfate esters on learning and memory in cognitive aging. Brain Res Brain Res Rev. (2001) 37 (1-3): 301-12. Review. Vallée M., et al.
4. Neurosteroids and potential therapeutics: Focus on pregnenolone. J Steroid Biochem Mol Biol. (2016) 160: 78-87. Review. Vallée M.

ADDITIONAL INFORMATION

• Full Name: Pregnenolone ELISA Kit
• Reactivity: Human
• Sample Type: Serum
• Sensitivity: 0.05 ng/ml

OTHER RELATED ELISA KITS

• DHEA ELISA Kit
• Progesterone ELISA Kit
• 17 OH Progesterone ELISA Kit