DHEA ELISA Kit (Dehydroepiandrosterone)
INTENDED USE
Human DHEA ELISA kit is a reliable quantitative procedure for measuring dehydroepiandrosterone (DHEA, androstenolone) in human serum and plasma (EDTA) samples. This assay has a minimum sensitivity detection of 0.07 ng/ml.
BACKGROUND
Dehydroepiandrosterone (DHEA, 3b-hydroxy-5-androsten-17-one, androstenolone) is predominately produced in the adrenal cortex and to a smaller amount in the gonads. It is a C19 steroid which serves as a precursor for the synthesis of testosterone and estrogen. There is literature to indicate that in adult women, peripubertal children and in neonates the levels that is circulating can be many fold higher than testosterone concentrations and that there is a possibility of a rapid peripheral tissue conversion to more potent androgens (such as testosterone and androstenedione) and estrogens.
Currently, the exact role of DHEA is not conclusive and there are a number of in vivo effects which have been reported such as enzyme-mediated cell metabolism and antiproliferative effects in different cell lines. There has been abnormal levels which have been identified in patients with obesity and schizophrenia, also therapeutic administration have been proposed for a number conditions like obesity and cardiovascular diseases. In vivo studies have indicated that it could affect insulin sensitivity and secretion, lipid and cholesterol metabolism and immune function.
The determination of serum DHEA has become a useful marker of adrenal androgen synthesis. Abnormally low have been identified in hypoadrenalism and elevated concentrations are found in many conditions such as: female hirsutism, 21-hydroxylase and 3b-hydroxysteroid dehydrogenase deficiencies and in most virilizing adrenal adenoma and carcinoma. Also due to the fact that very small amount is produced by the gonads, by determining the levels could help in localizing the androgen source in virilizing conditions.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Plate: Break apart strips coated with anti DHEA antibody.
- Enzyme Conjugate (100x Concentrate): Contains antibody conjugated to HRP.
- Standards A-F: Concentrations 0, 0.3, 1.0, 3.0, 10, 30 ng/ml. Avoid multiple freezing and thawing cycles.
- Control (High) And Control (Low).
- TMB Substrate Solution.
- Stop Solution: 1 M H2SO4.
- Wash Buffer Concentrate (10x).
SENSITIVITY
The minimum sensitivity detection limit of dehydroepiandrosterone (DHEA, androstenolone) using current DHEA ELISA kit was approximately 0.07 ng/ml. The dynamic range for this assay is 0.3 – 30.0 ng/ml.
ASSAY CHARACTERISTICS
– Analytical Sensitivity: 0.07 ng/ml
– Specificity (Cross Reactivity): DHEA (100%), Progesterone (0.23%), 17-Hydroxy-Pregnenolone (0.07%), Androstendione (0.06%), Desoxycorticosterone (0.05%), Pregnenolone (0.01%), DHEA-S, Testosterone, 5α-Dihydrotestosterone, 17α-Hydroxyprogesterone, Corticosterone, Cortisol (< 0.01%).
– Assay Dynamic Range: 0.3 – 30 ng/ml
– Intra-Assay Variation: 7.3 – 7.9%
– Inter-Assay Variation: 5.0 – 6.9%
– Recovery: 80 – 100%
– Linearity: 82 – 120%
– Drug Interferences: Any medication (cream, oil, pill etc.) containing DHEA will significantly influence the measurement of this analyte.
REFERENCES
- Dehydroepiandrosterone (DHEA): A fountain of youth? .Journal of Clinical Endocrinology and Meatabolism, (1996) 18: 3147. Baulieu, E.M.
- DHEA therapy for women: effect on sexual function and wellbeing. Hum Reprod Update. (2007) 13 (3): 239-48. Review. Panjari M. and Davis S.R.
ADDITIONAL INFORMATION
- Full Name: DHEA ELISA Kit (Dehydroepiandrosterone)
- Reactivity: Human
- Sample Type: Serum, Plasma (EDTA)
- Sensitivity: 0.07 ng/ml
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