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PIIINP ELISA Kit

Full Name: PIIINP ELISA Kit (N-terminal propeptide of procollagen type III)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.094 ng/ml

INTRODUCTION

PIIINP is an abbreviation, for N propeptide of collagen type III. It is a protein that gets separated during the creation of type III collagen. Collagen plays a role in providing support to connective tissues like skin, blood vessels and organs. By measuring PIIINP levels in the bloodstream valuable information can be obtained regarding collagen synthesis (tissue remodeling).

During the synthesis of collagen type III, PIIINP is produced alongside the collagen molecule. On it undergoes cleavage and enters circulation. The levels of PIIINP reflect the process of collagen type III biosynthesis occurring in tissues at a moment. Elevated levels suggest formation of collagen while decreased levels indicate production. Measuring PIIINP proves helpful in assessing tissue damage and fibrosis related to liver, kidney, lung and heart diseases.

Since skin and blood vessels contain amounts of collagen type III PIIINP, it serves as a marker for fibrotic skin conditions like scleroderma. It also aids in evaluating remodeling associated with atherosclerosis. In cases of liver fibrosis higher PIIINP levels correspond with stages of histological fibrosis. In terms of health elevated PIIINP indicates repair processes within myocardial tissue following an injury, whereas decreasing levels over time signify recovery. Moreover, elevated PIIINP may predict outcomes for individuals with heart failure. In lung fibrosis and chronic obstructive pulmonary disease (COPD) there exists a correlation between PIIINP levels and disease severity, as progression.

INTENDED USE

Human PIIINP ELISA kit can measure concentrations of N-terminal propeptide of procollagen type III (N-terminal propeptide collagen alpha-1(III) chain, PIIINP, Procollagen III Amino Terminal Propeptide, P3NP) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-PIIINP antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this PIIINP ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.094 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for PIIINP, no cross reactivity or interference between PIIINP and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (90 – 105%), Heparin Plasma (86 – 105%).
– Linearity: Serum (88 – 99%), EDTA Plasma (84 – 99%), Heparin Plasma (86 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. The aminoterminal propeptide of type III procollagen. Studies on physiology and pathophysiology. Dan Med Bull. (1997) 44 (1): 70-8. Jensen L.T.
  2. Procollagen assays in cancer. Adv Clin Chem. (2014) 66:79-100. Risteli L., et al.
  3. Aminoterminal propeptide of type III procollagen (PIIINP) is associated with ascending aortic aneurysm growth rate. Int J Cardiol. (2010) 145 (2): 379-380. Ormezzano O., et al.
  4. Spironolactone reduces brachial pulse wave velocity and PIIINP levels in hypertensive diabetic patients. Br J Clin Pharmacol. (2005) 59 (5): 520-3. Davies J., et al.

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