GPLD1 ELISA Kit

Full Name: GPLD1 ELISA Kit (Phosphatidylinositol-Glycan-Specific Phospholipase D)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.469 ng/ml

INTRODUCTION

The abbreviation GPLD1 refers to an enzyme called phosphatidylinositol glycan phospholipase D. This enzyme plays a role, in the breakdown of glycosylphosphatidylinositol (GPI) anchors, which attach proteins to the outer surface of the cell membrane. By cleaving these GPI anchors GPLD1 allows for the release of proteins from the cell surface. This process helps regulate signaling pathways and interactions between cells.

The GPLD1 gene is responsible for producing the GPLD1 enzyme, which is found throughout the body. Its main function is to break down the connection between GPI anchors and proteins allowing those anchored proteins to be released. Some examples of these anchored proteins include endothelin receptors, folate receptors and alkaline phosphatases. The activity of GPLD1 is influenced by fatty acids, which stimulate its function while cholesterol inhibits it. Additionally inflammatory cytokines can increase the expression of GPLD1.

By modulating the levels of GPI anchored proteins on cell surfaces GPLD1 affects processes such as adhesion, movement, signaling and immune function. For instance, it facilitates the release of receptors from cells resulting in reduced folate uptake. In cancer cases specifically elevated expression of GPLD1 contributes to metastasis by breaking down anchors that hold cadherins, in place. Overactive GPLD1 has also been observed in atherosclerosis.

INTENDED USE

Human GPLD1 ELISA kit can measure concentrations of phosphatidylinositol-glycan-specific phospholipase D (GPLD1, GPIPLD, GPIPLDM, PIGPLD, PIGPLD1) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-GPLD1 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this GPLD1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.781, 1.562, 3.125, 6.25, 12.5, 25, 50 ng/ml.
Reactivity: Human
Sensitivity: 0.469 ng/ml
Range: 0.781 – 50 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for GPLD1, no cross reactivity or interference between GPLD1 and analogues was detected.
– Recovery: Serum (93 – 102%), EDTA Plasma (85 – 103%), Heparin Plasma (86 – 105%).
– Linearity: Serum (88 – 105%), EDTA Plasma (92 – 100%), Heparin Plasma (81 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. The role of GPLD1 in chronic diseases. J Cell Physiol. (2023) 238 (7): 1407-1415. Cao J., et al.
2. High-Density Lipoprotein Carries Markers That Track With Recovery From Stroke. Circ Res. (2020) 127 (10): 1274-1287. Plubell D.L., et al.
3. New Horizon: Exercise and a Focus on Tissue-Brain Crosstalk. J Clin Endocrinol Metab. (2021) 106 (8): 2147-2163. Townsend L.K., et al.
4. Structure and expression of the human glycosylphosphatidylinositol phospholipase D1 (GPLD1) gene. Biochim Biophys Acta. (2000) 1494 (1-2): 189-94. Schofield J.N. and Rademacher T.W.

OTHER RELATED ELISA KITS

• NAMPT ELISA Kit
• PIIINP ELISA Kit
• NEFL ELISA Kit