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P-Cadherin (CDH3) ELISA Kit

INTENDED USE

Human P-cadherin ELISA kit can be used for analysing in vitro quantitative concentrations of P-cadherin (placental cadherin, cadherin-3, CDH3) in human serum, tissue homogneates, biological fluids and serum. This assay has a minimum sensitivity limit of 37.5 pg/ml.

BACKGROUND

P-Cadherin (also known as CDH3 or placental cadherin) has a major role in structural integrity conversation of epithelial tissues and it is a predominant calcium dependent glycoprotein which is essential for cell-cell adhesion. These are important for homophilic binding that can be vital in growth and development of cells through the many control mechanisms of tissue maintenance and architecture.

The cadeherin-3 (CDH3) gene has been identified to interact with CDH1, beta-catenin, nephrin, catenin alpha-1 and plakoglobin. It shares around 67 % of homology with the E-cadherin protein where the majority of difference can be found in the extracellular portion, however P-cadherin is less well characterized.

P-cadherin has been found to contribute to the biology of stem cells (e.g. normal mammary gland and hair follicle) and it has been reported to be present in in embryonic stem cells too. It is responsible for regulating many cellular homeostatic processes which are vital for embryonic development and in maintaining the architecture of adult tissues which can be important for cell shape, cell differentiation, cell polarity, cell growth and migration.

The expression of cadherin can be regulated either by spatially or temporally and recent findings have indicated that P-cadherin expression is crucial in maintaining architecture of normal breast epithelial cells. The loss or mutation of P-cadherin has been found to induce many characteristic genetic syndromes such as hypotrichosis with juvenile macular dystrophy (HJMD), ectrodactyly and macular dystrophy (EEM syndrome) and ectodermal dysplasia.

P-CADHERIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti CDH3 antibody.
  • Standards: Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml, made up from lyophilized recombinant human P-cadherin.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled CDH3 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of placental cadherin (cadherin-3, CDH3) using this human P-cadherin ELISA kit was 37.5 pg/ml. The dynamic range for this assay is 62.5 – 4,000 pg/ml.

ASSAY CHARACTERISTICS

– P-Cadherin (CDH3): ELISA
– Specificity: Highly specific for CDH3, no cross reactivity or interference between CDH3 and analogues was detected.
– Recovery: Serum (86 – 102%), EDTA Plasma (91 – 105%), Heparin Plasma (85 – 95%).
– Linearity: Serum (89 – 104%), EDTA Plasma (84 – 93%), Heparin Plasma (84 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Cadherins: The Superfamily Critically Involved in Breast Cancer. Curr Pharm Des. (2016) 22 (5): 616-38. Review. Ashaie M.A. and Chowdhury E.H.
  2. P-cadherin and the journey to cancer metastasis. Mol Cancer. (2015) 14: 178. Review. Vieira A.F. and Paredes J.
  3. Tuning the kinetics of cadherin adhesion. J Invest Dermatol. (2013) 133 (10): 2318-23. Review. Sivasankar S.
  4. Thinking outside the cell: how cadherins drive adhesion. Trends Cell Biol. (2012) 22 (6): 299-310. Review. Brasch J., et al.
  5. The role of P-cadherin in skin biology and skin pathology: lessons from the hair follicle. Cell Tissue Res. (2015) 360 (3): 761-71. Review. Samuelov L., et al.

ADDITIONAL INFORMATION

  • Full Name: P-Cadherin (CDH3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Tissue Homogenates, Biological Fluids, Serum
  • Sensitivity: 37.5 pg/ml

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