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OxLDL ELISA Kit (Oxidized Low-Density Lipoprotein)

Full Name: OxLDL ELISA Kit (Oxidized Low-Density Lipoprotein)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 1.875 ng/ml

INTRODUCTION

Oxidized LDL (OxLDL) is a form of LDL cholesterol that has been damaged by oxidation. LDL cholesterol is considered the “bad” cholesterol because it contributes to plaque buildup in arteries. The structure of OxLDL differs from native LDL in that the apolipoprotein B-100 (protein component) becomes oxidized, as do the phospholipids and cholesterol esters. This oxidative modification alters the function of OxLDL compared to native LDL. For example, oxidized LDL is taken up by scavenger receptors on macrophages, turning them into foam cells, which are the hallmark of early atherosclerotic lesions.

OxLDL inhibits nitric oxide production and promotes platelet activation and aggregation. It is not normally detected in plasma, but levels are elevated in patients with cardiovascular disease. Factors that generate reactive oxygen species, such as smoking, hypertension, and diabetes, increase oxidized LDL formation. Reducing exposure to these risk factors may decrease OxLDL production and atherosclerosis progression.

INTENDED USE

Human OxLDL ELISA kit can measure concentrations of oxidized low-density lipoprotein (OxLDL) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-OxLDL antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this OxLDL ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 3.125, 6.25, 12.5, 25, 50, 100, 200 ng/ml.
Reactivity: Human
Sensitivity: 1.875 ng/ml
Range: 3.125 – 200 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for OxLDL, no cross reactivity or interference between OxLDL and analogues was detected.
– Recovery: Serum (86 – 104%), EDTA Plasma (86 – 105%), Heparin Plasma (89 – 105%).
– Linearity: Serum (87 – 102%), EDTA Plasma (84 – 91%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. Exosomes derived from oxLDL-stimulated macrophages induce neutrophil extracellular traps to drive atherosclerosis. Cell Cycle. (2019) 18 (20): 2674-2684. Zhang Y.G., et al.
  2. Salidroside protects against ox-LDL-induced endothelial injury by enhancing autophagy mediated by SIRT1-FoxO1 pathway. BMC Complement Altern Med. (2019) 19 (1): 111. Zhu Z., Li J., Zhang X.
  3. The Oxidized Lipoproteins In Vivo: Its Diversity and Behavior in the Human Circulation. Int J Mol Sci. (2023) 24 (6): 5747. Itabe H, Obama T.
  4. Resveratrol and desferoxamine protect human OxLDL-treated granulosa cell subtypes from degeneration. J Clin Endocrinol Metab. (2014) 99 (1): 229-39. Schube U., et al.

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