Noradrenaline ELISA Kit (Norepinephrine)

INTENDED USE

Human noradrenaline ELISA kit is intended for measuring in vitro quantitative levels of noradrenaline or norepinephrine in human urine and plasma samples. This assay has a minimum detection limit of 1.7 ng/ml (U) and 0.04 pg/ml (P).

BACKGROUND

Noradrenaline (norepinephrine) is a member of the catecholamine family, it has vital functions within the brain and the body. Within the brain, norepinephrine is found to be formed in the nuclei or closely packed brain cell neurons which are yet too small to exert any meaningful effects on other areas of the brain. Outside the brain, it is primary functions as a neurotransmitter by sympathetic ganglia which is located in the abdomen or near the spinal cord, it can also be directly released into the bloodstream via the adrenal glands.

Noradrenaline acts on specific target cell by binding to and activating certain noradrenergic receptors which are located on the cell surface, regardless of where and how it is released. It has a structure is similar to epinephrine, the only difference is the presence of a methyl group attached to its nitrogen in epinephrine. A major function is to mobilise the body and in particular the brain for action. Within the brain, it is well known to promoting vigilance, increases arousal and alertness, focuses attention, increases restlessness or anxiety, enhances the formation and retrieval of memory.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Adhesive Foil.
• Wash Buffer (50x Concentrated).
• Enzyme Conjugate.
• Substrate: TMB Chromogenic.
• Stop Solution.
• Noradrenaline Microtiter Strips: Antigen pre-coated microwell plate.
• Antiserum – Rabbit anti-noradrenaline antibody.
• Adjustment Buffer.
• Standards (A-F): Concentrations 0, 5, 20, 75, 250, 1,000 ng/ml
• Acylation Buffer.
• Acylation Reagent.
• Assay Buffer.
• Coenzyme: S-adenosyl-L-methionine.
• Enzyme: Catechol-O-methyltransferase.
• Extraction Buffer.
• Extraction Plate.
• Hydrochloric Acid.
• Control 1.
• Control 2.

SENSITIVITY

The minimum sensitivity level of norepinephrine or noradrenaline using current noradrenaline ELISA kit was approximately 0.04 pg/ml (P) or 1.7 ng/ml (U). The dynamic range for this assay is 5.0 – 1,000 ng/ml.

ASSAY CHARACTERISTICS

– Expected Reference Values: < 90 µg/day (Urine) and < 600 pg/mL (Plasma)
– Analytical Sensitivity (Limit of Detection): 1.5 ng/mL (Urine) and 50 pg/mL (Plasma)
– Analytical Sensitivity: Derivatized Noradrenaline (100%), Normetanephrine 0.48%), Derivatized Dopamine (0.2%), Derivatized Adrenaline (0.14%), 3-Methoxy-4-hydroxyphenylglycol (0.01%), – Metanephrine, Tyrosine, 3-Methoxytyramine, Tyramine, 3-Methoxy-4-hydroxymandelic acid, Phenylalanine, Caffeinic acid, Homovanillic acid, L-Dopa (< 0.003%).
– Intra Assay Precision: 9.8 – 16.1%
– Inter Assay Precision: 8.5 – 15.0%
– Linearity: 85 – 123% (Urine) and 84 – 123% (Plasma)
– Recovery: 83 – 115% (Urine) and 85 – 108% (Plasma)

REFERENCES

1. Relationships between central nervous system noradrenergic function and plasma and urinary concentrations of norepinephrine metabolites. Adv Biochem Psychopharmacol. (1984) 39: 45-55. Review. Maas J.W.
2. Determination of catecholamines in plasma and urine. Best Pract Res Clin Endocrinol Metab. (2013) 27 (5): 713-23. Review. Grouzmann E. and Lamine F.
3. Assessment of O-methylated catecholamine levels in plasma and urine for diagnosis of autonomic disorders. Auton Neurosci. (2004) 116 (1-2): 1-10. Review. Oeltmann T., et al.
4. Recent advances in methods for the analysis of catecholamines and their metabolites. Anal Bioanal Chem. (2006) 386 (3): 506-14. Review. Tsunoda M.
5. The role of catecholamines in clinical medicine. Acta Med Scand Suppl. (1979) 624:9-18. Review. Christensen N.J.

ADDITIONAL INFORMATION

• Full Name: Noradrenaline ELISA Kit (Norepinephrine)
• Reactivity: Human
• Sample Type: Urine, Plasma
• Sensitivity: 17.0 ng/ml (U), 0.04 pg/ml (P)

OTHER RELATED ELISA KITS

• Adrenaline (Epinephrine) ELISA Kit
• Dopamine ELISA Kit
• 5-Hydroxy-3-Indole Acetic Acid (5-HIAA) ELISA Kit