Dopamine ELISA kit is designed for analysing in vitro quantitative concentrations of dopamine (hydroxytyramine) in plasma and urine samples. This assay has a minimum sensitivity detection limit of 1.9 ng/ml (plasma) and 2.5 ng/ml (urine).
Dopamine (hydroxytyramine) is a nitrogen containing organic compound which is produced as an intermediate compound from dihydroxyphenylalanine (dopa). It is one of the catecholamine neurotransmitters in the brain, which can be derived from tyrosine and is a precursor to adrenaline (epinephrine) and noradrenaline (norepinephrine). Dopamine has a major function as a neurotransmitter where it has the ability to inhibit the transmission of nerve impulses in basal ganglia, substantia nigra and the corpus striatum of the brain. There are a family of dopamine receptors which are responsible for mediating its action, where dopamine binds alpha-1 and beta-1 adrenergic receptors. This dopamine ELISA kit is designed to be only used for analysing plasma or urine samples.
Dopamine is a chemical in your brain that is responsible for affecting your emotions, movements, sensations of pleasure and pain. It is a neurotransmitter which is located in the deep middle region of the brain which is known as the substantia nigra. A deficiency of dopamine is linked with cellular death in the substantia nigra which can lead to Parkinson disease. Even any abnormalities in dopamine transmission, for example hyperactive dopamine transmission in certain parts of the brain, have been associated with psychotic syndromes (e.g. schizophrenia). However, dopaminergic structures within the brain (mainly the nucleus accumbens and the striatum) have been implicated in reward related behaviour.
This assay is based on a competitive procedure and dopamine has been extracted using a cis-diol-specific affinity gel, acylated and then enzymatically derivatized. Due to the fact that the chemical structure of catecholamines is identical in all animals and the performance of a pre-extraction step means that this kit is able to be used for a wide range of animal species.
DOPAMINE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Adhesive Foil.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate.
- Substrate (TMB).
- Stop Solution.
- Dopamine Antiserum.
- Adjustment Buffer.
- Standards (A-F): Concentrations 0, 10, 40, 150, 500, 2,000 ng/ml.
- Control 1.
- Control 2.
- Acylation Buffer.
- Acylation Reagent.
- Assay Buffer.
- Coenzyme: S-adenosyl-L-methionine.
- Enzyme (Lyophilized): Catechol-O-methyltransferase.
- Extraction Buffer.
- Extraction Plate (2x 48 well).
- Hydrochloric Acid.
- Dopamine Microtiter Strips: Antigen pre-coated microwell plate.
The minimum sensitivity detection limit of hydroxytyramine (dopamine) using current dopamine ELISA kit was approximately 2.5 ng/ml (U) and 1.9 ng/ml (P). The dynamic range for this assay is 10 – 2,000 ng/ml.
– Dopamine: ELISA
– Analytical Sensitivity: LOD – 2.5 ng/ml (Urine), 49 pg/ml (Plasma) and LOQ – 4.8 ng/ml (Urine), 75 pg/ml (Plasma).
– Analytical Specificity (Cross Reactivity): Derivatized Dopamine (100%), Derivatized Noradrenaline (6.4%), 3-Methoxytyramine (0.49%), Tyramine (0.18%), Normetanephrine (0.01%), Derivatized Adrenaline (0.02%), Metanephrine (< 0.01%), 3-Methoxy-4-hydroxyphenylglycol (< 0.01%), Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine, 3-Methoxy-4-hydroxymandelic acid (< 0.01%).
– Intra Assay Precision: 9.4 – 19.7% (Urine) and 24.4 – 29.8% (Plasma).
– Inter Assay Precision: 11.0 – 23.7% (Urine) and 14.2 – 28.2% (Plasma).
– Linearity: 83 – 126% (Urine) and 85 – 132% (Plasma).
– Recovery: 101 – 124% (Urine) and 84 – 92% (Plasma).
- Safety out of control: dopamine and defence. Behav Brain Funct. (2016) 12 (1): 15. Review. Lloyd K. and Dayan P.
- Dopamine signals and physiological origin of cognitive dysfunction in Parkinson’s disease. Mov Disord. (2015) 30 (4): 472-83. Review. Matsumoto M.
- Striatal dopamine, reward, and decision making in schizophrenia. Dialogues Clin Neurosci. (2016) 18 (1): 77-89. Review. Deserno L., et al.
- The Role of Dopamine and Its Dysfunction as a Consequence of Oxidative Stress. Oxid Med Cell Longev. (2016) 2016: 9730467. Review. Juárez Olguín H., et al.
- Protective and toxic roles of dopamine in Parkinson’s disease. J Neurochem. (2014) 129 (6): 898-915. Review. Segura-Aguilar J., et al.
- Full Name: Dopamine ELISA Kit
- Reactivity: Any Species
- Sample Type: Plasma, Urine
- Sensitivity: 1.9 ng/ml (P) and 2.5 ng/ml (U)