Adrenaline (Epinephrine) ELISA Kit
INTENDED USE
Human adrenaline ELISA kit is a reliable and accurate in vitro quantitative procedure for analysing adrenaline (epinephrine) in human plasma and urine samples. This assay has a minimum sensitivity detection limit of 0.01 pg/ml (P) and 0.8 ng/ml (U).
BACKGROUND
Epinephrine which is also referred to as adrenaline is a hormone that is primary secreted by the medulla of the adrenal glands. Strong emotions (fear or anger) are responsible for causing the adrenaline to be released into the bloodstream. This process results in increasing the heart rate, blood pressure, sugar metabolism and muscle strength and is often referred to as the “flight or fight response”. The main function of this response is to prepare the body for strenuous activity. Adrenaline (epinephrine) is derived from tyrosine amino acid and can be sometimes referred to as a catecholamine because it contains the catechol moiety.
The following epinephrine ELISA kit is a competitive assay where adrenaline (epinephrine) has been extracted through a cis-diol-specific affinity gel, acylated and then derivatized enzymatically. One of the main places in the body where epinephrine has an effect is in the liver, alongside glucagon, it is responsible for breaking down glycogen which is a form of energy storage in all animals. This is suitable for analsying various diagnostic application using either urine or plasma samples. Adrenaline can cause the smooth muscles to relax, especially those that are contained in the lungs. It is able to bind specifically to the beta-2-adrenergic receptors on bronchiole muscle cells and this causes the bronchioles to relax, which enables intensified respiration. In heart muscle cells epinephrine is able to bind beta-adrenergic receptors which results in increasing the contraction rate of the heart, this ultimately leading to elevated levels of blood supply to the tissues within the body. In medicine it has can be used for injections in order to relieve the symptoms of allergic reactions and can also be used in cardiopulmonary resuscitation (CPR).
ADRENALINE/EPINEPHRINE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Adhesive Foil.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate – Goat anti-rabbit immunoglobulins, conjugated with peroxidase.
- TMB Chromogenic Substrate.
- Stop Solution.
- Adrenaline Microtiter Strips.
- Adrenaline Antiserum
- Adjustment Buffer.
- Standards (A-F): Concentrations 0, 1, 4, 15, 50, 200 ng/mL.
- Control 1 and Control 2.
- Acylation Buffer.
- Acylation Reagent.
- Assay Buffer.
- Coenzyme: S-adenosyl-L-methionine.
- Enzyme (Lyophilized): Catechol-O-methyltransferase.
- Extraction Buffer.
- Extraction Plate.
- Hydrochloric Acid.
SENSITIVITY
The minimum sensitivity detection limit of adrenaline (epinephrine) using current human epinephrine ELISA kit was approximately 0.8 ng/ml (U) and 0.01 pg/ml (P). The dynamic range for this assay is 1 – 200 ng/ml.
ASSAY CHARACTERISTICS
– Adrenaline/Epinephrine: ELISA
– Expected Reference Value: < 20 µg/day (Urine) and < 100 pg/mL (Plasma).
– Analytical Sensitivity (Limit of Detection): 0.3 ng/mL (Urine) and 10 pg/mL.
– Analytical Specificity (Cross Reactivity): Derivatized Adrenaline (100%), Derivatized Noradrenaline (0.20%), Derivatized Dopamine (< 0.0007%), Metanephrine (0.64%), Normetanephrine (0.0009%), 3-Methoxytyramine (< 0.0007%), 3-Methoxy-4-hydroxyphenylglycol (0.03%), Tyramine (< 0.0007%), Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine, 3-Methoxy-4-hydroxymandelic acid (< 0.0007%).
– Intra Assay Precision: 6.9 – 15.0%
– Inter Assay Precision: 13.2 – 15.4%
– Linearity: 86 – 124% (Urine) and 81 – 121% (Plasma).
– Recovery: 84 – 119% (Urine) and 80 – 113% (Plasma).
REFERENCES
- Epinephrine biosynthesis: hormonal and neural control during stress. Cell Mol Neurobiol. (2006) 26 (4-6): 891-900. Review. Wong DL.
- Epinephrine and the metabolic syndrome. Curr Hypertens Rep. (2012) 14 (1): 1-7. Review. Ziegler M.G., et al.
- Stress and the adrenocortical control of epinephrine synthesis. Metabolism. (2002) 51 (6 Suppl 1): 11-4. Review. Wurtman RJ.
- Does nebulised adrenaline (epinephrine) reduce admission rate in bronchiolitis? Arch Dis Child. (2002) 87 (6): 548-50. Review. Meates M.
- Adrenaline: insights into its metabolic roles in hypoglycaemia and diabetes. Br J Pharmacol. (2016) 173 (9): 1425-37. Review. Verberne A.J., et al.
ADDITIONAL INFORMATION
- Full Name: Adrenaline (Epinephrine) ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Urine
- Sensitivity: 0.01 pg/ml (P) and 0.8 ng/ml (U)
