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Mouse TNF Alpha ELISA Kit (Tumor Necrosis Factor Alpha)

Full Name: Mouse TNF Alpha ELISA Kit (Tumor Necrosis Factor Alpha)
Reactivity: Mouse
Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
Sensitivity: 2.344 pg/ml

INTRODUCTION

Three identical subunits make up the multi-chain protein known as tumour necrosis factor alpha. Each of them has a transmembrane area, an internal region within the cell and a soluble section outside of it. ProTNF-alpha is formed from a precursor protein which has an N-terminal signal sequence together with a C-terminal component. The coiled coil domains help structure the protein into an alpha helix shape.

Macrophages, platelets, T helper cells, mast cells, and other cell types can all create TNF-alpha. It has a central role in inflammation. It was discovered in 1975 by U.S. scientists and has been linked to a number of autoimmune diseases, cardiovascular disorders and cancer. The levels present in human plasma are not associated with body mass index (BMI) per se. Its expression in adipose tissue is increased in obese individuals, as well as in the absence of obesity.

Through IL1 receptor antagonist (IL1Ra), TNF-alpha can trigger an inflammatory response in macrophages. This can result in the release of chemokines from macrophages, such as CCL2, which facilitates the attraction and activation of monocytes. The activation of monocytes causes the release of cytokines such as; TNF-alpha, IL-1beta and IL-6, this can trigger a series of inflammatory processes.

INTENDED USE

Mouse TNF alpha ELISA kit can be used for analysing amounts of tumor necrosis factor alpha (TNF alpha, TNFα, TNF-a) in mouse plasma, serum, biological fluids and tissue homogenates samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with TNF alpha antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

The following mouse TNF alpha ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 3.9, 7.81, 15.6, 31.25, 62.5, 125, 250 pg/ml.
Reactivity: Mouse
Sensitivity: 2.344 pg/ml
Range: 3.906 – 250 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for TNF-alpha, no cross reactivity or interference between TNF-alpha and analogues was detected.
– Recovery: Serum (95 – 105%), EDTA Plasma (86 – 101%), Heparin Plasma (92 – 103%).
– Linearity: Serum (87 – 93%), EDTA Plasma (85 – 101%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. The TNF-alpha transgenic mouse model of inflammatory arthritis. Springer Semin Immunopathol. (2003) 25 (1): 19-33. Li P., et al.
  2. Methylmercury induces the expression of TNF-α selectively in the brain of mice. Sci Rep. (2016) 6: 38294. Iwai-Shimada M., et al.
  3. Tumor necrosis factor-alpha (TNF-alpha) stimulates proliferation of mouse primordial germ cells in culture. Dev Biol. (1994) 161 (1): 91-5. Kawase E., et al.

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