Mouse Lymphotoxin Alpha ELISA Kit (LT-Alpha)

Full Name: Mouse Lymphotoxin Alpha ELISA Kit (LT-Alpha)
Reactivity: Mouse
Sample Type: Serum, Plasma, Biological Fluids, Tissue Homogenates
Sensitivity: 9.375 pg/ml

INTRODUCTION

Lymphotoxin alpha (LT-alpha) is composed of a bundle of three helices that form a triangular shape. The protein is 233 amino acids, it has a molecular weight of 26 kDa and has a disulfide bond between residues 58 and 185. It is produced by activated lymphocytes, this can include T cells and natural killer cells. Other cells such as epithelial cells are also known to produce it. The protein is found in nearly all vertebrates and has a 20% frequency. It has been identified in human peripheral blood cells, inflammatory sites, and intracellular compartments. LT-alpha is also understood to serve a number of purposes. TNF-alpha is thought to specifically attach to it, turning it into an inactive version.

Lymphotoxin alpha regulates the development and organisation of lymphoid tissues. It plays a role in the formation of secondary lymphoid organs (for example lymph nodes and spleen) and it helps to maintain the integrity of blood-lymph barriers (gut-associated lymphoid tissue). Other roles of LT-alpha include; the recruitment of immune cells to sites of infection or injury, pro-inflammatory effects and to be involved in the development of autoimmune (rheumatoid arthritis). LFA-1 regulates integrins and several other proteins involved in cell adhesion, migration and signalling pathways. These proteins include chemokines CXCL12, CXC3, IL8R and VLA4. The interaction between LT-alpha and its receptor LFA-1 is vital for lymphocyte activation (proliferation) which is necessary to mount an immune response when faced with an infection or injury by foreign materials such as toxins or pathogens.

INTENDED USE

Mouse Lymphotoxin alpha ELISA kit is designed for measuring concentration of lymphotoxin-alpha (LT-alpha, lymphotoxin alpha) present in mouse plasma, serum, biological fluids or tissue homogenates.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with LT alpha
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

The following mouse lymphotoxin alpha ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.6, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Mouse
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for LT-alpha, no cross reactivity or interference between LT-alpha and analogues was detected.
– Recovery: Serum (87 – 105%), EDTA Plasma (85 – 98%), Heparin Plasma (92 – 103%).
– Linearity: Serum (85 – 103%), EDTA Plasma (85 – 103%), Heparin Plasma (92 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Lymphotoxin-alpha contributes to lymphangiogenesis. (2010) 116 (12): 2173-82. Mounzer R.H., et al.
2. Lymphotoxin-α promotes tumor angiogenesis in HNSCC by modulating glycolysis in a PFKFB3-dependent manner. Int J Cancer. (2019) 145 (5): 1358-1370. Yang J.G., et al.
3. Controlling herpetic stromal keratitis by modulating lymphotoxin-alpha-mediated inflammatory pathways. Microbes Infect. 2013 Sep-Oct;15(10-11):677-87. Veiga-Parga T., et al.

OTHER RELATED ELISA KITS

• Mouse TNF Alpha ELISA Kit
• TGF-Beta ELISA Kit
• Mouse MCP-1 ELISA Kit