Mouse MCP-1 ELISA Kit (Monocyte Chemotactic Protein 1)

Full Name: Mouse MCP-1 ELISA Kit (Monocyte Chemotactic Protein 1)
Reactivity: Mouse
Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma
Sensitivity: 9.375 pg/ml

INTRODUCTION

Monocyte chemotactic protein 1 is a chemokine that is produced by macrophages, monocytes and other cells of the immune system. It is involved in the recruitment of monocytes to sites of injury and inflammation. It is also responsible for promoting both neutrophil recruitment to sites of injury/infection and T lymphocyte proliferation. This aids in triggering the signal transduction pathway necessary for T cell activation. MCP-1 attaches to the CCR2 chemokine receptor, which is present on the surface of numerous immune cell subtypes.

Infectious pathogens, reactive radicals, and pro-inflammatory mediators generated by host cells all cause an increase in MCP-1. The main physiological job is to mediate host defence. It triggers cells to release cytokines and superoxide, which enhances macrophage phagocytosis and antigen presentation while also luring monocytes to the diseased or injured location.

MCP-1 and IL-8 both have comparable expression patterns. Both cytokines will rise in response to LPS activation and inflammatory cytokines such IL-1, TNF-a, and IFN-g. In addition to being chemotactic for monocytes, It also activates mouse monocytes to become cytostatic for several mouse tumour cell lines, release lysosomal enzymes, and generate superoxide. Elevated MCP-1 levels are associated with; atherosclerosis, glomerulonephritis, autoimmune disease, allergic inflammation and granuloma formation.

INTENDED USE

Mouse MCP-1 ELISA kit can detect amounts of monocyte chemotactic protein 1 (MCP-1, CCL2, GDF-2, HC11, MCAF, MCP1, SMC-CF) present in mouse serum, plasma, tissue homogenates and various biological fluids.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with antibody MCP-1.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this mouse MCP-1 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Mouse
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for MCP-1, no cross reactivity or interference between MCP-1 and analogues was detected.
– Recovery: Serum (92 – 97%), EDTA Plasma (86 – 104%), Heparin Plasma (895 – 104%).
– Linearity: Serum (85 – 105%), EDTA Plasma (94 – 104%), Heparin Plasma (85 – 101%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Invest. (2006) 116 (6): 1494-505. Kanda H., et al.
2. Curcumin inhibits in vitro MCP-1-release from mouse pancreatic islets. Transplant Proc. (2006) 38 (9): 3035-8. Amoli M.M., et al.
3. Inhibition of MCP-1/CCR2 signaling does not inhibit intimal proliferation in a mouse aortic transplant model. J Vasc Res. (2008) 45 (6): 538-46. Alexis J.D., et al.
4. An antagonist of monocyte chemoattractant protein 1 (MCP-1) inhibits arthritis in the MRL-lpr mouse model. J Exp Med. (1997) 186 (1): 131-7. Gong J.H., et al.

OTHER RELATED ELISA KITS

• Mouse IL-23 ELISA Kit
• TGF-Beta 2 ELISA Kit
• TGF-Beta ELISA Kit