Mouse IL-8/CXCL15 ELISA Kit (Interleukin 8)

Full Name: Mouse IL-8/CXCL15 ELISA Kit (Interleukin 8)
Reactivity: Mouse
Sample Type: Biological Fluids, Serum, Plasma, Tissue Homogenates
Sensitivity: 9.375 pg/ml

INTRODUCTION

An individual of the CXC family of chemokines is interleukin 8. This is owing to the amino terminal region having two conserved cysteine residues that are only one amino acid apart from one another. CXCL15 is created from a precursor of 99 amino acids, which is subsequently changed into the mature form of 72 amino acids. Four non-glycosylated cysteine residues make up the mature form, which can be secreted. from cells or proteins. There is no change in the structure of IL-8 between different species. This is owing to the amino terminal region having two conserved cysteine residues that are only one amino acid apart from one another. IL-8 is involved in the inflammatory and immune responses of these cells.

As a novel biomarker of inflammation, IL-8 has also been said to be particularly helpful in identifying pulmonary conditions such chronic inflammatory and fibrotic lung illnesses. It is a crucial chemical messenger for triggering an active immune response since it has been discovered to be very effective at drawing basophils, neutrophils and T lymphocytes. Due to its consistent accuracy and efficacy, CXCL15 has been utilised frequently in the medical research community as a biomarker to gauge the severity of a specific inflammatory response. It can be used as a marker to control cardiovascular disorders and acute respiratory problems. This is supported by the findings that levels are elevated in both healthy individuals and those with an active infection.

INTENDED USE

Mouse IL-8/CXCL15 ELISA kit can be used for determining concentrations of interleukin-8 (CXCL15, IL-8, lungkine, Scyb15, Weche) present mouse in tissue homogenates, plasma, other biological fluids and serum samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti IL-8 antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this mouse IL-8/CXCL15 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Mouse
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-8/cxcl15, no cross reactivity or interference between interleukin-8 and analogues was detected.
– Recovery: Serum (85 – 103%), EDTA Plasma (85 – 102%), Heparin Plasma (85 – 101%).
– Linearity: Serum (85 – 98%), EDTA Plasma (86 – 99%), Heparin Plasma (84 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

CXCL15/Lungkine has suppressive activity on proliferation and expansion of multi-potential, erythroid, granulocyte and macrophage progenitors in S-phase specific manner. J.Blood Cells Mol Dis. (2021) 91: 102594. Broxmeyer H.E., et al.
IL-8 mRNA expression in the mouse mammary glands during pregnancy and lactation. Genet Mol Res. (2012) 11 (4): 4746-53. Li H.P., et al.
Circulating CXC-chemokine concentrations in a murine intestinal ischemia-reperfusion model. Fetal Pediatr Pathol. (2004) 23 (2-3): 145-57. Maheshwari A., et al.
Expression of CXCL15 (Lungkine) in murine gastrointestinal, urogenital, and endocrine organs. J Histochem Cytochem. (2007) 55 (5): 515-24. Schmitz J.M., et al.

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