Mouse IL-1 Beta ELISA Kit (Interleukin-1 Beta)
INTENDED USE
Mouse IL-1 beta ELISA kit is ideal for detecting levels of interleukin-1 beta (IL-1b, IL-1 beta, IL-1beta) in mouse samples of plasma, serum, tissue homogenate and many other biological fluids.
INTRODUCTION
Interleukin 1 is composed of two of proteins namely IL-1 alpha and IL-1 beta, there are separate gene that are responsible for producing each of these proteins. Macrophages and other cells have the capacity to produce IL-1 in response to events like inflammation, infections, or microbial endotoxins. This leads to a drastic raise in its production and release. Compared to other cytokines, IL-1 has few key functions. It also plays a key role in chronic inflammation as well as acute inflammation that responds to such natural events like infection or tissue injury.
IL-1 alpha and IL-1 beta are closely related proteins; they are composed of similar amino acids, sharing around 25% similarity. Both proteins are initially synthesized as 31kDa precursors, but then get cleaved into smaller proteins with molecular weights of 17.5kDa. In cells, IL-1 beta is only present in the 31 kilodalton (kDa) precursor form. When found outside of cells, it is made up of a combination of the unprocessed and mature forms. IL-1 beta is derived primarily from macrophages and activated T cells. It plays a role in fever and sepsis as an acute cytokine. It also suppresses lymphocyte proliferation via the p38 Mitogen Activated Protein Kinase pathway.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-IL-1b antibody.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this mouse interleukin-1 beta ELISA kit it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 12.5, 25, 50, 100, 200, 400, 800 pg/ml.
Reactivity: Mouse
Sensitivity: 7.5 pg/ml
Range: 12.5 – 800 pg/ml
Principle: Sandwich
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for IL-1 Beta, no cross reactivity or interference between IL-1 Beta and analogues was detected.
– Recovery: Serum (96 – 101%), EDTA Plasma (94 – 103%), Heparin Plasma (89 – 96%).
– Linearity: Serum (85 – 104%), EDTA Plasma (84 – 92%), Heparin Plasma (81 – 94%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
REFERENCES
- Re-exposure to alarmin HMGB1 and cytokine IL-1_beta induces differential innate immune response generation in mouse brain. J Neuroimmunol. (2021) 357: 577625. Das S., et al.
- Expression of IL-1β and implantation serine proteases is required for mouse blastocyst hatching. Reproduction. (2021) 161 (2): 123-133. Pathak M., et al.
- Interleukin-1 beta targeted therapy for type 2 diabetes. Expert Opin Biol Ther. (2009) (9): 1177-88. Maedler K., et al.
- Inhibition of IL-1beta improves Glycaemia in a Mouse Model for Gestational Diabetes. Sci Rep. (2020) 10 (1): 3035. Schulze F., et al.
ADDITIONAL INFORMATION
- Full Name: Mouse IL-1 Beta ELISA Kit (Interleukin-1 Beta)
- Reactivity: Mouse
- Sample Type: Biological Fluids, Plasma, Tissue Homogenates, Serum
- Sensitivity: 7.5 pg/ml
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