MCP-3 ELISA Kit (Monocyte Chemotactic Protein 3)
INTENDED USE
MCP-3 ELISA kit can be used to determine in vitro quantitative levels of monocyte chemotactic protein 3 (MCP3, CCL7, MCP-3) in human serum, plasma, cell culture supernatant and many other biological fluids. This assay has a minimum detection limit of < 30.0 pg/ml.
BACKGROUND
Monocyte chemotactic protein 3 (MCP3) is now called chemokine (C-C motif) ligand 7 (CCL7), it is purified from human osteosarcoma cell line. Due to the fact that it contains two adjacent N-terminal cysteine residues in its mature protein, it is classified as a CC chemokines subfamily member. It has the ability to bind and activate a large number of diverse inflammatory cell types via the interaction with multiple leukocyte receptors as well as it’s own receptor. Evidence indicates that it does indeed use multiple receptors (which includes MCP-1, MIP-1a, RANTES) and can also bind with MIP-1beta receptor as well as other unique binding sites. It can specifically attracts monocytes and regulates macrophage function. CCL7 has been shown to interact with MMP2.
cDNA cloning of MCP-3 and structural analysis has revealed that it composed of 76 amino acid polypeptide with a molar mass of 8.5 kDa. It is part of a small family of inflammatory proteins that can be characterized by four conserved cysteine residues and it is located on the human chromosome 17. MCP-3 and MCP-1 display 71% a.a. homology, whilst it is also revealed to have 30% a.a homology with RANTES and MIP-1 and these all can activate T-cells, basophils, monocytes and eosinophils. It can also promote the exocytosis of eosinophil granule proteins and stimulates the release of histamine from human basophils.
SENSITIVITY
The minimum detection sensitivity level of monocyte chemotactic protein 3 (MCP3, MCP-3, CCL7) using current MCP-3 ELISA kit was 30.0 pg/ml. The dynamic range for this assay is 30.0 – 1,600 pg/ml.
REFERENCES
- Cytokines and chemoattractants in allergic inflammation. Mol Immunol. (2002) 38 (12-13): 881-5. Review. Romagnani S.
- Monocyte chemotactic protein-3. Eur Cytokine Netw. (2001) 12 (4): 554-60. Review. Menten P., et al.
- CC chemokines in allergic inflammation. Immunol Today. (1994) 15 (3): 127-33. Review. Baggiolini M. and Dahinden C.A.
- The role of CXCL12 and CCL7 chemokines in immune regulation, embryonic development, and tissue regeneration. Cytokine. (2014) 69 (2): 277-83. Review. Cheng J.W., et al.
ADDITIONAL INFORMATION
- Full Name: MCP-3 ELISA Kit (Monocyte Chemotactic Protein 3)
- Reactivity: Human
- Sample Type: Plasma, Serum, Cell Culture Supernatant, Biological Fluids
- Sensitivity: < 30.0 pg/ml
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