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MCP-1 ELISA Kit (Monocyte Chemotactic Protein 1)

INTENDED USE

Human MCP-1 ELISA kit is a protocol intended for analysing in vitro quantitative amounts of monocyte chemotactic protein 1 (MCP-1)(MCAF, LDCF, GDF) in human biological fluids, serum, plasma and tissue homogenates. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Monocyte chemotactic protein 1 (MCP1, MCP-1) which is also known as; MCAF, GDF and LDCF. It is composed of a 76-amino acid polypeptide with an approx. molecular mass of 8,700 Da and it belongs to a family low molecular weight structurally-related proteins that are characterised by four conserved cysteine residues (known as C-C or intercrine b family).

MCP-1 is expressed by many cultured cells such as; lymphocytes, endothelial cells, monocytes, smooth muscle cells, fibroblasts and many transformed cell lines upon stimulation with cytokines or LPS. In addition to being chemotactic for monocytes, it can also activate human monocytes in order to become cytostatic for many human tumour cell lines, generate superoxide and release lysosomal enzymes. In order to further clarify and elucidate the participation and relation of MCAF with various disorders, an in vivo quantitative analysis of this protein is necessary.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human MCP-1 antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-Labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of monocyte chemotactic protein 1 (MCP 1, MCAF) using this MCP-1 ELISA kit was 9.375 pg/ml. The dynamic range for this assay is 15.625 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for MCP 1, no cross reactivity or interference between MCP 1 and analogues was detected.
– Recovery: Serum (85 – 103%), EDTA Plasma (89 – 97%), Heparin Plasma (87 – 103%).
– Linearity: Serum (89 – 105%), EDTA Plasma (82 – 100%), Heparin Plasma (92 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Impact of genetic variations in C-C chemokine receptors and ligands on infectious diseases. Hum Immunol. (2016) 77 (10): 961-71. Review. Qidwai T. and Khan M.Y.
  2. Interleukin-8 and MCAF: novel leukocyte recruitment and activating cytokines. Chem Immunol. (1992) 51: 236-65. Review. Matsushima K., et al.
  3. Monocyte Chemoattractant Protein 1 (MCP-1) in obesity and diabetes. Cytokine. (2012) 60 (1): 1-12. Review. Panee J.
  4. Renaming cytokines: MCP-1, major chemokine in pancreatitis. Gut. (2005) 54 (12): 1679-81. Review. Marra F.

ADDITIONAL INFORMATION

  • Full Name: MCP-1 ELISA Kit (Monocyte Chemotactic Protein 1)
  • Reactivity: Human
  • Sample Type: Tissue Homogenates, Plasma, Biological Fluids, Serum
  • Sensitivity: 9.375 pg/ml

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