MMP-7 ELISA Kit (Matrix Metalloproteinase-7)

INTENDED USE

Human MMP-7 ELISA kit is a method designed for detecting in vitro quantitative amounts of matrix metalloproteinase-7 (MMP7, MMP-7, matrilysin, pump-1 protease, uterine metalloproteinase) in human serum, cell culture supernatant, cell lysates, other biological fluids and plasma. This assay has a minimum sensitivity limit of 0.094 ng/ml.

BACKGROUND

Matrix metalloproteinase-7 (MMP-7, MMP7, matrilysin, uterine metalloproteinase or pump-1 protease), consists of structural-related zinc-dependent endopeptidases. It is a protein which is bound by 4 metal ions (including catalytic zinc ion), 2 calcium ions and a structural zinc ion. It is to break-down extracellular matrix using macromolecules such as casein, proteoglycan, fibronectin and gelatins. The protein itself is usually secreted as zymogen and the prodoamin contains approx. 9 kD of highly conserved cysteine switch that is located near the C-terminal containing cysteine residues.

MMP-7 is identified to be potentially associated with tumor metastasis and a number inflammatory processes. It has the ability to cleave cell surface proteins and to promote the adhesion of cancer cells and therefore increase the likely hood of tumor metastasis.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with MMP-7.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of matrix metalloproteinase-7 (MMP7, MMP-7, uterine metalloproteinase, matrilysin, pump-1 protease) using current MMP-7 ELISA kit was 0.094 ng/ml. The dynamic range for this assay is 0.156 – 10 ng/ml.

REFERENCES

1. Matrilysin (matrix metalloproteinase-7): a new promising drug target in cancer and inflammation? Cytokine Growth Factor Rev. (2004) 15 (2-3): 111-5. Review. Wielockx B., et al.
2. MT1-MMP and MMP-7 in invasion and metastasis of human cancers. Cancer Metastasis Rev. (2003) 22 (2-3): 145-52. Review. Shiomi T. and Okada Y.
3. Biomarkers in idiopathic pulmonary fibrosis. Curr Opin Pulm Med. (2012) 18 (5): 441-6. Review. Zhang Y. and Kaminski N.
4. Matrilysin as a target for chemotherapy for colon cancer: use of antisense oligonucleotides as antimetastatic agents. Cancer Chemother Pharmacol. (1999) 43 Suppl: S52-5. Review. Miyazaki K., et al.

ADDITIONAL INFORMATION

• Full Name: MMP-7 ELISA Kit (Matrix Metalloproteinase-7)
• Reactivity: Human
• Sample Type: Other Biological Fluids, Plasma, Cell Lysates, Cell Culture Supernatant, Serum
• Sensitivity: 0.094 ng/ml

OTHER RELATED ELISA KITS

• MMP-3 ELISA Kit
• MMP-9 ELISA Kit
• MMP-14 ELISA Kit