Matrix Metalloproteinase-2 (MMP-2) ELISA Kit
INTENDED USE
Human MMP-2 ELISA kit is a protocol for measuring in vitro quantitative concentrations of matrix metalloproteinase-2 (MMP-2, MMP2, type IV collagenase or gelatinase A) in human cell culture supernatant, plasma, cell lysates, serum and many other biological fluids. This assay has a minimum sensitivity limit of 0.243 ng/mL.
BACKGROUND
Matrix metalloproteinase-2 (MMP2, MMP-2) is also called gelatinase A or type IV collagenase (72 kDa) and is located at position 12.2 on chromosome 16. It plays a major function in arteriogenesis and angiogenesis, both of these processes are essential in restoring tissue perfusion after ischemia. There are evidence to indicate that the expression of MMP-2 is elevated in tissue ischemia, however the mechanism which is responsible is still not well understood. A proteolytic process is required in order to activate MMP-2, cell-cell clustering of integrin chains can also promote activation.
The following MMP-2 ELISA kit is intended for analysing many different types of diagnostic applications. Matrix metalloproteinase-2 can interact with Thrombospondin 1, TIMP2, TIMP4, CCL7 and THBS2. It is known to play crucial roles in the formation of newly formed blood vessels in tumors (angiogenesis) and this is essential for tumor progression since it provides a supply of nutrients and oxygen. Finally, MMPs can be discovered to proteolytically activate TGF-beta, this is important in promoting epithelial mesenchymal transition, a process which is vital during cancer metastasis. This MMP-2 ELISA kit offers a sesnitivity level of 0.243 ng/mL and it can be used to measure human samples of cell lysates, cell culture supernatant, other biological fluids, plasma and serum.
SENSITIVITY
The minimum detection sensitivity level of matrix metalloproteinase-2 (MMP2, MMP-2, gelatinase A and type IV collagenase) using current human MMP-2 ELISA kit was 0.243 ng/mL. The dynamic range for assay is 0.625 – 40 ng/mL.
REFERENCES
- Targeting MMP-2 to treat ischemic heart injury. Basic Res Cardiol. (2014) 109 (4): 424. Review. Hughes B.G. and Schulz R.
- The response of matrix metalloproteinase-9 and -2 to exercise. Sports Med. (2015) 45 (2): 269-78. Nascimento Dda C., et al.
- Matrix metalloproteinases in exercise and obesity. Vasc Health Risk Manag. (2016) 12: 287-95. Review. Jaoude J. and Koh Y.
- Myocardial matrix metalloproteinase-2: inside out and upside down. J Mol Cell Cardiol. (2014) 77: 64-72. Review. DeCoux A., et al.
ADDITIONAL INFORMATION
- Full Name: Matrix Metalloproteinase-2 (MMP-2) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Cell Culture Supernatant, Cell Lysates, Other Biological Fluids, Plasma
- Sensitivity: 0.243 ng/mL
