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MMP-2 ELISA Kit (Matrix Metalloproteinase-2)

INTENDED USE

Human MMP-2 ELISA kit is a protocol for measuring in vitro quantitative concentrations of matrix metalloproteinase-2 (MMP-2, MMP2, type IV collagenase or gelatinase A) in human cell culture supernatant, plasma, cell lysates, serum and many other biological fluids. This assay has a minimum sensitivity limit of 0.191 ng/ml.

BACKGROUND

Matrix metalloproteinase-2 (MMP2, MMP-2) is also called gelatinase A or type IV collagenase (72 kDa) and is located at position 12.2 on chromosome 16. It plays a major function in arteriogenesis and angiogenesis, both of these processes are essential in restoring tissue perfusion after ischemia. A proteolytic process is required in order to activate MMP2, cell-cell clustering of integrin chains can also promote activation.

MMP-2 can interact with; Thrombospondin 1, TIMP2, TIMP4, CCL7 and THBS2. It is known to play crucial roles in the formation of newly formed blood vessels in tumors (angiogenesis) and this is essential for tumor progression since it provides a supply of nutrients and oxygen. MMPs can proteolytically activate TGF-beta, this is important in promoting epithelial mesenchymal transition, a process which is vital during cancer metastasis.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with MMP-2.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of human matrix metalloproteinase-2 (MMP2, MMP-2, gelatinase A and type IV collagenase) using current MMP-2 ELISA kit was 0.191 ng/ml. The dynamic range for assay is 0.5 – 32 ng/ml.

REFERENCES

  1. Targeting MMP-2 to treat ischemic heart injury. Basic Res Cardiol. (2014) 109 (4): 424. Review. Hughes B.G. and Schulz R.
  2. The response of matrix metalloproteinase-9 and -2 to exercise. Sports Med. (2015) 45 (2): 269-78. Nascimento Dda C., et al.
  3. Matrix metalloproteinases in exercise and obesity. Vasc Health Risk Manag. (2016) 12: 287-95. Review. Jaoude J. and Koh Y.
  4. Myocardial matrix metalloproteinase-2: inside out and upside down. J Mol Cell Cardiol. (2014) 77: 64-72. Review. DeCoux A., et al.

ADDITIONAL INFORMATION

  • Full Name: MMP-2 ELISA Kit (Matrix Metalloproteinase-2)
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant, Cell Lysates, Other Biological Fluids, Plasma
  • Sensitivity: 0.191 ng/ml

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