MMP-10 ELISA Kit (Matrix Metalloproteinase-10)

INTENDED USE

Human MMP-10 ELISA kit is a method intended for analysing in vitro quantitative levels of matrix metalloproteinase-10 (MMP-10, MMP10, transin-2, stromelysin-2) in human plasma, cell lysates, serum, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 46.875 pg/ml.

BACKGROUND

Matrix metalloproteinase-10 (MMP10, stromelysin-2, MMP-10 or transin-2) is found to degrade a wide rand of substances such as collagen (III, IV, V), elastin, aggrecan, gelatin, laminin, cartilage proteoglycan and fibronectin. It is expressed in T cells, keratinocytes, menstrual endometrium and some tumor samples. It has also been associated with cancer stem cell metastasis and vitality.

MMP-10 protein can separated into four distinct domains: a catalytic domain with a zinc binding site, short linker region, carboxyl terminal hemopexin-like domain and a pro-domain which is cleaved upon activation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with MMP-10.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of human matrix metalloproteinase-10 (MMP10, MMP-10, stromelysin-2, transin-2) using current MMP-10 ELISA kit was 46.875 pg/ml. The dynamic range for this assay is 78.125 – 5000 pg/ml.

REFERENCES

1. Interplay of extracellular matrix and leukocytes in lung inflammation. Cell Immunol. (2017) 312: 1-14. Review. Wight T.N., et al.
2. Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. (2015) 53 (5): 585-600. Review. Craig V.J., et al.
3. Metalloproteinases and atherothrombosis: MMP-10 mediates vascular remodeling promoted by inflammatory stimuli. Front Biosci. (2008) 13: 2916-21. Review. Rodriguez JA, et al.
4. Extracellular matrix mediates a molecular balance between vascular morphogenesis and regression. Curr Opin Hematol. (2008) 15 (3): 197-203. Review. Davis G.E. and Senger D.R.

ADDITIONAL INFORMATION

• Full Name: MMP-10 ELISA Kit (Matrix Metalloproteinase-10)
• Reactivity: Human
• Sample Type: Cell Lysates, Serum, Other Biological Fluids, Cell Culture Supernatant, Plasma
• Sensitivity: 46.875 pg/ml

OTHER RELATED ELISA KITS

• MMP-1 ELISA Kit
• MMP-2 ELISA Kit
• MMP-11 ELISA Kit

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