Matrix Metalloproteinase-10 (MMP-10) ELISA Kit
INTENDED USE
Human MMP-10 ELISA kit is a method intended for analysing in vitro quantitative levels of matrix metalloproteinase-10 (MMP-10, MMP10, transin-2, stromelysin-2) in human plasma, cell lysates, serum, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 25.0 pg/mL.
BACKGROUND
Matrix metalloproteinase-10 (MMP10, stromelysin-2, MMP-10 or transin-2) is found to degrade a wide rand of substances such as collagen (III, IV, V), elastin, aggrecan, gelatin, laminin, cartilage proteoglycan and fibronectin. MMP-10 is expressed in T cells, keratinocytes, menstrual endometrium and some tumor samples. The following MMP-10 ELISA kit can be used for analysing various diagnostic applications. It has also been associated with cancer stem cell metastasis and vitality. Recent evidence indicates that MMP-10 is a secreted metalloproteinases that has major roles in skeletal development, vascular remodelling and wound healing, however, its overexpression has been implicated in tumor progression and lung tumorigenesis.
The gene is located on chromosome 11q22.3 where it is part of a cluster of MMP genes. The MMP-10 protein can separated into four distinct domains: a catalytic domain with a zinc binding site, short linker region, carboxyl terminal hemopexin-like domain and a pro-domain which is cleaved upon activation. This MMP-10 ELISA kit offers a sensitivity level of 25.0 pg/mL and it can be used to measure human samples of serum, biological fluids, plasma, cell culture supernatant and cell lysates.
SENSITIVITY
The minimum detection sensitivity level of matrix metalloproteinase-10 (MMP10, MMP-10, stromelysin-2, transin-2) using current human MMP-10 ELISA kit was 25.0 pg/mL. The dynamic range for this assay is 78.125 – 5,000 pg/mL.
REFERENCES
- Interplay of extracellular matrix and leukocytes in lung inflammation. Cell Immunol. (2017) 312: 1-14. Review. Wight T.N., et al.
- Matrix metalloproteinases as therapeutic targets for idiopathic pulmonary fibrosis. Am J Respir Cell Mol Biol. (2015) 53 (5): 585-600. Review. Craig V.J., et al.
- Metalloproteinases and atherothrombosis: MMP-10 mediates vascular remodeling promoted by inflammatory stimuli. Front Biosci. (2008) 13: 2916-21. Review. Rodriguez JA, et al.
- Extracellular matrix mediates a molecular balance between vascular morphogenesis and regression. Curr Opin Hematol. (2008) 15 (3): 197-203. Review. Davis G.E. and Senger D.R.
ADDITIONAL INFORMATION
- Full Name: Matrix Metalloproteinase-10 (MMP-10) ELISA Kit
- Reactivity: Human
- Sample Type: Cell Lysates, Serum, Other Biological Fluids, Cell Culture Supernatant, Plasma
- Sensitivity: 25.0 pg/mL
