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Rat LRG ELISA Kit (Leucine Rich Alpha-2-Glycoprotein)

Full Name: Rat LRG ELISA Kit (Leucine Rich Alpha-2-Glycoprotein)
Reactivity: Rat
Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
Sensitivity: 1.68 ng/ml

BACKGROUND

Leucine rich alpha-2-glycoprotein (LRG) is a protein with a lot of leucine repeats in it. It is crucial for a variety of physiological activities, including as cell adhesion, immunological control and inflammatory response. LRG research sheds light on how our bodies react to sickness and how it might be used as a diagnostic and therapeutic tool. It has different molecular shapes and sizes, depending on its location and function within cells. It can have alternating regions of hydrophobic and hydrophilic regions that are organized in concentric layers.

Cell surface LRGs are the first line of defence, being 2-5 nm long and 10-50 nm wide. They act as an interface between the external cell environment and the internal cellular environment. The hydrophilic regions of these LRGs form part of the external leaflet of the plasma membrane, which is exposed to intercellular aqueous media such as bodily fluids and cytoplasm. This allows for rapid transport between cells and water. It is hydrophobic and inhibits water loss through transpiration. The protein-conducting channel of the human sperm flagellum is a complex network of interconnected LRG proteins. It includes the motor proteins, which have one central LRG surrounded by six other LRGs. The surrounding LRRGs aid in propulsion by creating holes in ECM as they rotate. This leads to hydroplaning off the outer layer of ECM, which is then re-coated. Flagella are only effective in the water and therefore they must be constantly replaced by new ones.

INTENDED USE

Rat LRG ELISA kit can be used for qualitative measurement of leucine rich alpha-2-glycoprotein (leucine rich alpha-2-glycoprotein, LRG) using rat serum, urine, culture supernatant and EDTA-plasma samples. This assay has a minimum sensitivity detection limit of 1.68 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Pre-Coated Plate: Anti-rat LRG (191) rabbit IgG.
  • Labelled antibody (30x Conc.).
  • Standard: Recombinant.
  • EIA Buffer.
  • Solution For Labelled Antibody.
  • Chromogen (TMB).
  • Wash Buffer (40x Conc.).
  • Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of leucine rich alpha-2-glycoprotein (LRG, leucine rich alpha-2-glycoprotein) using current rat LRG ELISA kit is 1.68 ng/ml. The dynamic range for this assay is 6.25 – 500 ng/ml.

ASSAY CHARACTERISTICS

– Added Recovery Assay: 83.2 – 110.3%
– Intra – Assay: 5.6 – 7.0%
– Inter – Assay: 5.1 – 11.7%
– Sensitivity: 1.68 ng/mL

REFERENCES

  1. Novel brain-specific bovine cDNA for a developmentally regulated mRNA encoding a putative new member of the leucine-rich glycoprotein (LRG) family. Neurochem Res. (1992) 17 (9): 907-16. Tan E.C. and Lim L.
  2. Myeloid cell-derived LRG attenuates adverse cardiac remodelling after myocardial infarction. Cardiovasc Res. (2016) 109 (2): 272-82. Kumagai S., et al.
  3. Leucine-rich α-2-glycoprotein promotes TGFβ1-mediated growth suppression in the Lewis lung carcinoma cell lines. Oncotarget. (2015) 6 (13): 11009-22. Takemoto N., et al.
  4. LRG-accelerated differentiation defines unique G-CSFR signaling pathways downstream of PU.1 and C/EBPepsilon that modulate neutrophil activation. J Leukoc Biol. (2008) 83 (5): 1277-85. Ai J., et al.

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