Mouse LRG ELISA Kit (Leucine Rich Alpha-2-Glycoprotein)

Full Name: Mouse LRG ELISA Kit (Leucine Rich Alpha-2-Glycoprotein)
Reactivity: Mouse
Sample Type: Plasma (EDTA), Serum, Cell Culture Supernatant
Sensitivity: 0.06 ng/ml

BACKGROUND

Leucine repeats are abundant in the protein known as leucine rich alpha-2-glycoprotein (LRG). It is essential for several physiological processes, including as cell adhesion, immune system regulation and inflammatory response. Leucine repeats are also found in the protein known as leucine rich repeat domain-containing protein (LRRD). It is involved in processes including; cell adhesion, development of the central nervous system and formation of muscle tissue. It is connected to diseases including cancer and Alzheimer’s. LRG research helps us comprehend how our bodies react to disease and how it might be employed as a diagnostic and therapeutic tool.

Depending on where it is located and what it does for cells, LRG takes on a variety of molecular forms and sizes. LRG can exist as: A small lipid molecule that is only a few nanometers long. A large molecule, typically 500-1500 nanometers long. An LRG’s tail is about 15 to 30 nanometers long, while the globular head has a diameter of 100 to 200 nanometers. Multiple small molecules, with lengths of 100 to 150 nanometers, attached in parallel to the head and tail by another molecule (likely glycerol) that is only 10-20 nanometers long can exist.

LRG may be composed of layers of concentric hydrophobic and hydrophilic zones that alternate. LRGs come in three main categories. While type A and type B LRGs are found in the Golgi apparatus, type C LRGs typically appear inside vacuoles or on the surface of cells. Type C LRGs consist of a surface membrane that surrounds an intracellular chamber and an outer membrane. LRGs are generally found in the cytosol and may be composed of layers of concentric hydrophobic and hydrophilic zones that alternate.

INTENDED USE

Mouse LRG ELISA kit is designed for analysing qualitative levels of leucine rich alpha-2-glycoprotein (alpha-2-glycoprotein, LRG) in mouse serum, cell culture supernatant and plasma. This assay has a minimum sensitivity detection limit of 0.06 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti-mouse LRG (138) Rabbit IgG.
• Labelled antibody (30x Conc.).
• Standard: Recombinant mouse leucine rich alpha2-glycoprotein.
• EIA Buffer.
• Solution for Labelled antibody.
• Chromogen (TMB).
• Stop Solution.
• Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of mouse leucine rich alpha-2-glycoprotein (LRG, alpha-2-glycoprotein) using current mouse LRG ELISA kit is 0.06 ng/ml. The dynamic range for this assay is 0.25 – 16.0 ng/ml.

ASSAY CHARACTERISTICS

– Added Recovery Assay: 76.9 – 100.0%
– Intra – Assay: 5.7 – 9.0%
– Inter – Assay: 5.3 – 11.0%
– Sensitivity: 0.06 pg/mL

REFERENCES

1. Lrg participates in lipopolysaccharide preconditioning-induced brain ischemia injury via TLR4 signaling pathway. J Mol Neurosci. (2014) 54 (1): 20-6. Gong G., et al.
2. ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand. J Immunol Methods. (2008) 336 (1): 22-9. Weivoda S., et al.
3. Leucine-rich α-2-glycoprotein_promotes TGFβ1-mediated growth suppression in the Lewis lung carcinoma cell lines. Oncotarget. (2015) 6 (13): 11009-22. Takemoto N., et al.
4. Leucine rich α-2 glycoprotein is a potential urinary biomarker for renal tubular injury. Biochem Biophys Res Commun. (2018) 498 (4): 1045-1051. Lee H., et al.

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