Rat COX-2 ELISA Kit (Cyclooxygenase-2)

Full Name: Rat COX-2 ELISA Kit (Cyclooxygenase-2)
Reactivity: Rat
Sample Type: Tissue Lysates, Cell Lysates
Sensitivity: 0.16 ng/ml

BACKGROUND

Cyclooxygenase-2 is necessary for prostaglandins, that is required in a several physiological processes. Prostaglandin G/H Synthase 2 (PGHS2) is another name for it and because it is membrane-bound, it can only operate on molecules that are close to its active site. COX2 is essential for tissue healing, pain perception and inflammation. Its potential therapeutic benefit in treating conditions including cancer and arthritis has been thoroughly researched.

Tissues produce COX-2, which is a catalyst in the production of inflammatory mediators. Its expression is tightly regulated and can be induced through various cytokines, growth factors and endotoxins. This process helps to regulate inflammation, thereby providing protection against injury or disease. Functionally, it is a homodimer that is responsible for catalysing two steps during the conversion process of arachadonic acid into prostaglandin H2.

COX-2 is further subdivided into a number of COX isoenzymes. These different isoenzymes have distinct functional properties and are thought to be largely responsible for the differences in response to various stimuli observed. While COX-2 is found in white blood cells and platelets, COX-1 is located in high concentrations in the brain and heart. Both are involved in inflammation, but COX-2 is considered the main culprit behind pain and inflammation.

INTENDED USE

Rat COX-2 ELISA kit can be used for detecting quantitative levels of cyclooxygenase-2 (COX2, COX-2) in rat lysates of tissues or cells. This assay has a minimum sensitivity detection limit of 0.16 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
Pre-Coated Plate.
Labelled Antibody (30x Conc.).
HRP Conjugated Anti-Rat COX-2 (2h3).
Standard.
EIA Buffer.
Solution For Labelled Antibody.
TMB Chromogen.
Wash Buffer (40x Conc.).

SENSITIVITY

The minimum sensitivity detection limit of cyclooxygenase-2 (COX2) using current rat COX-2 ELISA kit was approximately 0.16 ng/ml. The dynamic range for this assay is 0.31 – 20.0 ng/ml.

ASSAY CHARACTERISTICS

– Titer Assay: 58.9 – 103.1%
– Added Recovery Assay: 53.1 – 75.5%
– Intra – Assay: 3.7 – 6.7%
– Inter – Assay: 6.8 – 9.9%
– Sensitivity: 0.16 ng/mL

REFERENCES

BDNF and COX-2 participate in anti-depressive mechanisms of catalpol in rats undergoing chronic unpredictable mild stress. Physiol Behav. (2015) 151: 360-8. Wang J.M., et al.
Does miRNA-155 Promote Cyclooxygenase-2 Expression in Cancer? Drug Dev Res. (2015) 76 (7): 354-6. Review. Comer B.S.
Cyclooxygenase 2: its regulation, role and impact in airway inflammation. Clin Exp Allergy. (2016) 46 (3): 397-410. Review. Rumzhum N.N. and Ammit A.J.
COX-2 inhibitors: a novel strategy in the management of breast cancer. Drug Discov Today. (2016) 21 (4): 598-615. Review. Regulski M., et al.

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