Human IL-8 ELISA kit is intended for measuring in vitro quantitative levels of interleukin-8 (IL-8) present in human serum, tissue homogenates, serum and other biological fluids. This assay has a minimum detection limit of 18.75 pg/ml.
Interleukin-8 (IL-8) which can also be known as T lymphocyte chemotactic factor (TCF), monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil attractant/activating protein (NAP-1), leukocyte adhesion inhibitor (LAI) and monocyte-derived neutrophil-activating peptide (MONAP). IL-8 belongs to a chemokine superfamily that has the ability to selectively activate and chemo-attract specific leukocyte subpopulations, all of these are cytokines which contain four conserved cysteines and can be divided into two distinguishable subfamilies (C-X-C proteins or CC-protein).
IL-8 is a member of the C-X-C subfamily alongside platelet basic protein (PBP), neutrophil-activating peptide-2 (NAP-2), platelet factor 4 (PF4), g-interferon-inducible protein (g-IP-10), connective-tissue-activating peptide III (CTAPIII), MGSA/CRO gene products (GRO-a, GRO-b, GRO-g) and b-thromboglobulin, ENA-78. Interleukin-8 is found to be produced by a number of cells in response to inflammatory stimuli examples include TNF-a, IL-1b, mitogen, viruses, lectins, phorbol esters (PMA) and crystals.
There are many cell types which are producing IL-8 in response to these stimuli such as fibroblasts, endothelial cells, neutrophils, keratinocytes, chondrocytes, mesothelial cells, glioblatoma cells, T lymphocytes, monocytes and macrophages. There are also various non-infectious human diseases which are linked with neutrophilia and neutrophil infiltration into organs. Some examples of these human diseases include immune vasculitis, psoriasis, rheumatoid arthritis, glomerulonephritis, gouty arthritis, inflammatory bowel disease, acute meningococcal infections, cystic fibrosis and alcoholic hepatitis.
IL-8 ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human IL-8 antibody.
- Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant human interleukin-8.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled IL-8 Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
The minimum detection sensitivity level of interleukin-8 (IL-8) using this human IL-8 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.
– Interleukin-8 (IL-8): ELISA
– Specificity: Highly specific for IL-8, no cross reactivity or interference between IL-8 and analogues was detected.
– Recovery: Serum (85 – 105%), EDTA Plasma (86 – 102%), Heparin Plasma (92 – 100%).
– Linearity: Serum (88 – 101%), EDTA Plasma (83 – 98%), Heparin Plasma (85 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
- Interleukin 8 and acute lung injury. Arch Pathol Lab Med. (2014) 138 (2): 266-9. Review. Allen T.C. and Kurdowska A.
- Roles of IL-8 in ocular inflammations: a review. Ocul Immunol Inflamm. (2011) 19 (6): 401-12. Review. Ghasemi H., et al.
- Interleukin-8 and human cancer biology. Cytokine Growth Factor Rev. (2001) 12 (4): 375-91. Review. Xie K.
- Mitogenic effects of interleukin-8/CXCL8 on cancer cells. Future Oncol. (2005) 1 (5): 699-704. Review. Zhu Y.M. and Woll P.J.
- Neutrophil receptors for interleukin-8 and related CXC chemokines. Semin Hematol. (1997) 34 (4): 311-8. Review. Murphy P.M.
- Full Name: Interleukin-8 (IL-8) ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma (Heparin, EDTA, Citrate), Other Biological Fluids, Tissue Homogenates
- Sensitivity: 18.75 pg/ml