Human IL-16 ELISA kit is a procedure for detecting in vitro quantitative amounts of interleukin-16 (IL-16) in human plasma, tissue homogenates, biological fluids and serum. This assay has a minimum detection limit of 18.75 pg/ml.
Interleukin-16 (IL-16) which was also previously known as lymphocyte chemoattractant factor (LCF), is essentially a lymphocyte chemo-attractant factor of T cell origin that as a selective activity for CD4+ T cells. IL-16 is a product of eosinophils, CD4+ cells, CD8+ cells, epithelial cells derived from asthmatics and mast cells. The expression of IL-16 in asthmatic epithelium is found to directly correlate with the number of infiltrating CD4+ T cells. In addition to its chemotactic activity, it is also a competence growth factor that is selective for CD4+ T cells that are dependent on an interaction with CD4 in order to induce their of functional activity.
IL-16 cDNA is known to code for a novel 14-kDa protein, which needs to form a homo-tetrameric complex in order to induce biological activity. In culture supernatants IL-16 appears as a relative molecular mass (Mr) of approx. 56,000 biologically active, non-covalently linked tetramer, but is found to migrate in monomeric form in SDS PAGE. The eluted monomeric peptides are inactive but have the ability to re-aggregate to Mr 56,000 which allows them to regain biological activity. The protein that is expressed from the IL-16 cDNA is able to demonstrate all the functions and chemical features of the native protein.
IL-16 ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human IL-16 antibody.
- Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant human interleukin-16.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled IL-16 Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
The minimum detection sensitivity level of interleukin-16 (IL-16) using this human IL-16 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.
– Interleukin-16 (IL-16): ELISA
– Specificity: Highly specific for IL-16, no cross reactivity or interference between IL-16 and analogues was detected.
– Recovery: Serum (85 – 101%), EDTA Plasma (87 – 104%), Heparin Plasma (92 – 105%).
– Linearity: Serum (86 – 101%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
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- CD8+ T-cell-mediated non-cytolytic suppression of human immuno-deficiency viruses. Curr Drug Targets Infect Disord. (2003) 3 (2): 97-113. Review. Vella C. and Daniels R.S.
- lnterleukin-16: the ins and outs of regulating T-cell activation. Crit Rev Immunol. (2008) 28 (6): 467-83. Review. Cruikshank W. and Little F.
- Prospects for IL-16 in the treatment of AIDS. Expert Opin Biol Ther. (2001) 1 (3): 425-32. Review. Kornfeld H. and Cruikshank W.W.
- Full Name: Interleukin-16 (IL-16) ELISA Kit
- Reactivity: Human
- Sample Type: Tissue Homogenates, Plasma, Serum, Biological Fluids
- Sensitivity: 18.75 pg/ml