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Interleukin-16 (IL-16) ELISA Kit

INTENDED USE

Human IL-16 ELISA kit is a procedure for detecting in vitro quantitative amounts of interleukin-16 (IL-16) in human plasma, tissue homogenates, biological fluids and serum. This assay has a minimum detection limit of 18.75 pg/ml.

BACKGROUND

Interleukin-16 (IL-16) which was also previously known as lymphocyte chemoattractant factor (LCF), is essentially a lymphocyte chemo-attractant factor of T cell origin that as a selective activity for CD4+ T cells. IL-16 is a product of eosinophils, CD4+ cells, CD8+ cells, epithelial cells derived from asthmatics and mast cells. The expression of IL-16 in asthmatic epithelium is found to directly correlate with the number of infiltrating CD4+ T cells. In addition to its chemotactic activity, it is also a competence growth factor that is selective for CD4+ T cells that are dependent on an interaction with CD4 in order to induce their of functional activity.

IL-16 cDNA is known to code for a novel 14-kDa protein, which needs to form a homo-tetrameric complex in order to induce biological activity. In culture supernatants IL-16 appears as a relative molecular mass (Mr) of approx. 56,000 biologically active, non-covalently linked tetramer, but is found to migrate in monomeric form in SDS PAGE. The eluted monomeric peptides are inactive but have the ability to re-aggregate to Mr 56,000 which allows them to regain biological activity. The protein that is expressed from the IL-16 cDNA is able to demonstrate all the functions and chemical features of the native protein.

IL-16 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-16 antibody.
  • Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant human interleukin-16.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-16 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-16 (IL-16) using this human IL-16 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-16 (IL-16): ELISA
– Specificity: Highly specific for IL-16, no cross reactivity or interference between IL-16 and analogues was detected.
– Recovery: Serum (85 – 101%), EDTA Plasma (87 – 104%), Heparin Plasma (92 – 105%).
– Linearity: Serum (86 – 101%), EDTA Plasma (82 – 93%), Heparin Plasma (80 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Interleukin-16 network in inflammation and allergy. Allergy Asthma Proc. (2002) 23 (2): 103-8. Review. Conti P., et al.
  2. Not-so-sweet sixteen: the role of IL-16 in infectious and immune-mediated inflammatory diseases. J Interferon Cytokine Res. (2006) 26 (8): 511-20. Review. Glass W.G., et al.
  3. Signaling and functional properties of interleukin-16. Int Rev Immunol. (1998) 16 (5-6): 523-40. Review. Cruikshank W.W., et al.
  4. CD8+ T-cell-mediated non-cytolytic suppression of human immuno-deficiency viruses. Curr Drug Targets Infect Disord. (2003) 3 (2): 97-113. Review. Vella C. and Daniels R.S.
  5. lnterleukin-16: the ins and outs of regulating T-cell activation. Crit Rev Immunol. (2008) 28 (6): 467-83. Review. Cruikshank W. and Little F.
  6. Prospects for IL-16 in the treatment of AIDS. Expert Opin Biol Ther. (2001) 1 (3): 425-32. Review. Kornfeld H. and Cruikshank W.W.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-16 (IL-16) ELISA Kit
  • Reactivity: Human
  • Sample Type: Tissue Homogenates, Plasma, Serum, Biological Fluids
  • Sensitivity: 18.75 pg/ml

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