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Interleukin-3 (IL-3) ELISA Kit

INTENDED USE

Human IL-3 ELISA kit is a procedure for analysing in vitro quantitative levels of interleukin-3 (IL-3) in human plasma (heparin, EDTA, citrate), tissue homogenates, serum and other biological fluid samples. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Interleukin-3 (IL-3) is essentially a hematopoietic colony-stimulating factor (CSF) which has the ability to support the proliferation of a large number of hematopoietic cell types. It is a protein that is 140 amino acids in length which is chemically synthesised using an automated peptide synthesiser and this displays biological activities that can be attributed to native IL-3.

The gene IL-3 in known to encode interleukin 3, where the human gene encoding IL-3 is found to be tandemly arrayed on the long arm of chromosome 5. The mouse interleukin-3 gene is located on chromosome 11 and the cDNA sequence for codes for a polypeptide of 166 amino acids including a putative signal peptide. It is regarded as one of the colony stimulating factors that is vital in regulating haematopoiesis.

IL-3 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-3 antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant human interleukin-3.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-3 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of IL-3 (interleukin-3) using current human IL-3 ELISA kit was approximately 9.375 pg/ml. The dynamic range for this assay is 15.625 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-3 (IL-3): ELISA
– Specificity: Highly specific for IL-3, no cross reactivity or interference between IL-3 and analogues was detected.
– Recovery: Serum (88 – 99%), EDTA Plasma (86 – 105%), Heparin Plasma (90 – 102%).
– Linearity: Serum (86 – 104%), EDTA Plasma (85 – 101%), Heparin Plasma (81 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Interleukin-3: structure and function. Cancer Invest. (1993) 11 (5): 609-23. Review. Lindemann A. and Mertelsmann R.
  2. Present and future clinical relevance of interleukin 3. Stem Cells. (1994) 12 Suppl 1: 241-8. Review. Gianella-Borradori A.
  3. Cytokines, interleukin-3 and autoimmunity. Isr J Med Sci. (1993) 29 (2-3): 159-64. Review. Fishman P. and Shoenfeld Y.
  4. Human interleukin 3: analysis of the gene and its role in the regulation of hematopoiesis. Int J Cell Cloning. (1990) 8 Suppl 1: 121-8. Review. Yang Y.C and Clark S.C.
  5. Interleukin-3 and its receptor. Cancer Treat Res. (1995) 80: 107-42. Review. Lindemann A. and Mertelsmann R.
  6. Interleukin 3: from colony-stimulating factor to pluripotent immunoregulatory cytokine. Int J Immunopharmacol. (1992) 14 (3): 421-30. Review. Frendl G.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-3 (IL-3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Biological Fluids, Serum, Plasma (EDTA, Citrate, Heparin), Tissue Homogenates
  • Sensitivity: 9.375 pg/ml

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