Interleukin-1 Beta (IL-1 BETA) ELISA Kit


Human IL-1 beta ELISA kit is intended for detecting in vitro quantitative concentrations of interleukin-1 beta (IL-1 beta, IL-1β, IL-1b) present in human tissue homogenates, serum, plasma (citrate, EDTA, heparin) other biological fluids. This assay has a minimum detection limit of 2.344 pg/ml.


Interleukin-1 beta (IL-1 beta, IL-1β or IL-1b) has been strongly implicated in the pathogenesis of osteoporosis and high bone turnover, it is a potent stimulator of bone resorption. Early researchers even called IL-1 beta endogenous pyrogen since it was able to induce fever and contributed to the wasting of muscles (PIF or proteolysis inducing factor). Low levels of IL-1 beta have been reported in normal serum and IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor. These inhibitors are structurally more similar to IL-1 beta and have been thought to play vital roles in regulating the action of IL-1 beta.

The cells that are known to express IL-1b include endothelial cells, natural killer (NK) cells, trophoblasts, astrocytes, osteoblasts, keratinocytes, adrenal cortical cells, Schwann cells, neutrophils, oligodendroglia, T cells plus fibroblasts, monocytes and macrophages, platelets and megakaryocytes. Interleukin-1β can cause nuclear export of a specific NCOR co-repressor complex, leading to the de-repression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. It is a prominent microglia-derived cytokine which can cause oligodendrocyte death in co-culture with astrocytes and microglia, but not in pure culture of oligodendrocytes by alone.

Furthermore, co-operation of IL-1 beta and PDGFB can result in inducing contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Micro-environmental IL-1β is essential for invasiveness and in vivo angiogenesis of different tumor cells.  This association with the disease can be used to explain the biologic properties of IL-1 beta, thereby making it an important pro-inflammatory cytokine and a powerful inhibitor of gastric acid secretion.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-1 beta antibody.
  • Standards: Concentrations 0, 3.906, 7.812, 15.625, 31.25, 62.5, 125, 250 pg/ml, made up from lyophilized recombinant human interleukin-1 beta.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-1 beta Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


The minimum detection sensitivity level of IL-1 beta (interleukin-1 beta, IL-1β, IL-1b) using current human interleukin-1 beta ELISA kit was approximately 2.344 pg/ml. The dynamic range for this assay is 3.906 – 250 pg/ml.


– Interleukin-1 Beta (IL-1 Beta): ELISA
– Specificity: Highly specific for IL-1 beta, no cross reactivity or interference between IL-1 beta and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (85 – 101%), Heparin Plasma (85 – 102%).
– Linearity: Serum (86 – 95%), EDTA Plasma (89 – 101%), Heparin Plasma (88 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.


  1. Effect of interleukin 1β inhibition in cardiovascular disease. Curr Opin Lipidol. (2012) 23 (6): 548-53. Review. Qamar A. and Rader D.J.
  2. Interleukin-1 beta: a potential link between stress and the development of visceral obesity. BMC Physiol. (2012) 12: 8. Review. Speaker K.J. and Fleshner M.
  3. A critical role for interleukin-1β in the progression of autoimmune diseases. Int Immunopharmacol. (2013) 17 (3): 658-69. Review. Zhao R., et al.
  4. Targeting interleukin-1β for pain. CNS Neurol Disord Drug Targets. (2011) 10 (5): 571-5. Review. Pillarisetti S.
  5. The interleukin 1beta pathway in the pathogenesis of osteoarthritis. J Rheumatol. (2008) 35 (12): 2306-12. Review. Daheshia M. and Yao J.Q.


  • Full Name: Interleukin-1 Beta (IL-1 Beta) ELISA Kit
  • Reactivity: Human
  • Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma (EDTA, Heparin, Citrate)
  • Sensitivity: 2.344 pg/ml



Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford