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Interleukin-1 Beta (IL-1 BETA) ELISA Kit

INTENDED USE

Human IL-1 beta ELISA kit is intended for detecting in vitro quantitative concentrations of interleukin-1 beta (IL-1 beta, IL-1β, IL-1b) present in human tissue homogenates, serum, plasma (citrate, EDTA, heparin) other biological fluids. This assay has a minimum detection limit of 2.344 pg/ml.

BACKGROUND

Interleukin-1 beta (IL-1 beta, IL-1β or IL-1b) has been strongly implicated in the pathogenesis of osteoporosis and high bone turnover, it is a potent stimulator of bone resorption. Early researchers even called IL-1 beta endogenous pyrogen since it was able to induce fever and contributed to the wasting of muscles (PIF or proteolysis inducing factor). Low levels of IL-1 beta have been reported in normal serum and IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor. These inhibitors are structurally more similar to IL-1 beta and have been thought to play vital roles in regulating the action of IL-1 beta.

The cells that are known to express IL-1b include endothelial cells, natural killer (NK) cells, trophoblasts, astrocytes, osteoblasts, keratinocytes, adrenal cortical cells, Schwann cells, neutrophils, oligodendroglia, T cells plus fibroblasts, monocytes and macrophages, platelets and megakaryocytes. Interleukin-1β can cause nuclear export of a specific NCOR co-repressor complex, leading to the de-repression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. It is a prominent microglia-derived cytokine which can cause oligodendrocyte death in co-culture with astrocytes and microglia, but not in pure culture of oligodendrocytes by alone.

Furthermore, co-operation of IL-1 beta and PDGFB can result in inducing contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Micro-environmental IL-1β is essential for invasiveness and in vivo angiogenesis of different tumor cells.  This association with the disease can be used to explain the biologic properties of IL-1 beta, thereby making it an important pro-inflammatory cytokine and a powerful inhibitor of gastric acid secretion.

IL-1 BETA ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-1 beta antibody.
  • Standards: Concentrations 0, 3.906, 7.812, 15.625, 31.25, 62.5, 125, 250 pg/ml, made up from lyophilized recombinant human interleukin-1 beta.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-1 beta Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of IL-1 beta (interleukin-1 beta, IL-1β, IL-1b) using current human interleukin-1 beta ELISA kit was approximately 2.344 pg/ml. The dynamic range for this assay is 3.906 – 250 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-1 Beta (IL-1 Beta): ELISA
– Specificity: Highly specific for IL-1 beta, no cross reactivity or interference between IL-1 beta and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (85 – 101%), Heparin Plasma (85 – 102%).
– Linearity: Serum (86 – 95%), EDTA Plasma (89 – 101%), Heparin Plasma (88 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Effect of interleukin 1β inhibition in cardiovascular disease. Curr Opin Lipidol. (2012) 23 (6): 548-53. Review. Qamar A. and Rader D.J.
  2. Interleukin-1 beta: a potential link between stress and the development of visceral obesity. BMC Physiol. (2012) 12: 8. Review. Speaker K.J. and Fleshner M.
  3. A critical role for interleukin-1β in the progression of autoimmune diseases. Int Immunopharmacol. (2013) 17 (3): 658-69. Review. Zhao R., et al.
  4. Targeting interleukin-1β for pain. CNS Neurol Disord Drug Targets. (2011) 10 (5): 571-5. Review. Pillarisetti S.
  5. The interleukin 1beta pathway in the pathogenesis of osteoarthritis. J Rheumatol. (2008) 35 (12): 2306-12. Review. Daheshia M. and Yao J.Q.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-1 Beta (IL-1 Beta) ELISA Kit
  • Reactivity: Human
  • Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma (EDTA, Heparin, Citrate)
  • Sensitivity: 2.344 pg/ml

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