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IL-1 Beta ELISA Kit (Interleukin-1 Beta)

INTENDED USE

Human IL-1 beta ELISA kit is intended for detecting in vitro quantitative concentrations of interleukin-1 beta (IL-1 beta, IL-1β, IL-1b) present in human tissue homogenates, serum, plasma (citrate, EDTA, heparin) other biological fluids. This assay has a minimum detection limit of 2.344 pg/ml.

BACKGROUND

Interleukin-1 beta (IL-1 beta, IL-1β or IL-1b) was originally called IL-1 beta endogenous pyrogen since it was able to induce fever and contributed to the wasting of muscles (PIF or proteolysis inducing factor). Low levels have been reported in normal serum and IL-1 inhibitors that vary only in their degree of glycosylation have been described to bind to the IL-1 receptor.

Co-operation of IL-1 beta and PDGFB results in inducing contractile to synthetic phenotype modulation of human aortic smooth muscle cells in culture. Micro-environmental is essential for invasiveness and in vivo angiogenesis of different tumor cells.  This association with the disease can be used to explain the biologic properties of IL-1 beta, thereby making it an important pro-inflammatory cytokine and a powerful inhibitor of gastric acid secretion.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-1 beta antibody.
  • Standards: Concentrations 0, 3.906, 7.812, 15.625, 31.25, 62.5, 125, 250 pg/ml, made up from lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-1 beta Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of IL-1 beta (interleukin-1 beta, IL-1β, IL-1b) using current IL-1 beta ELISA kit was approximately 2.344 pg/ml. The dynamic range for this assay is 3.906 – 250 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-1 beta, no cross reactivity or interference between IL-1 beta and analogues was detected.
– Recovery: Serum (85 – 104%), EDTA Plasma (85 – 101%), Heparin Plasma (85 – 102%).
– Linearity: Serum (86 – 95%), EDTA Plasma (89 – 101%), Heparin Plasma (88 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Effect of interleukin 1β inhibition in cardiovascular disease. Curr Opin Lipidol. (2012) 23 (6): 548-53. Review. Qamar A. and Rader D.J.
  2. Interleukin-1 beta: a potential link between stress and the development of visceral obesity. BMC Physiol. (2012) 12: 8. Review. Speaker K.J. and Fleshner M.
  3. A critical role for interleukin-1β in the progression of autoimmune diseases. Int Immunopharmacol. (2013) 17 (3): 658-69. Review. Zhao R., et al.
  4. The interleukin 1 beta pathway in the pathogenesis of osteoarthritis. J Rheumatol. (2008) 35 (12): 2306-12. Review. Daheshia M. and Yao J.Q.

ADDITIONAL INFORMATION

  • Full Name: IL-1 Beta ELISA Kit (Interleukin-1 Beta)
  • Reactivity: Human
  • Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma (EDTA, Heparin, Citrate)
  • Sensitivity: 2.344 pg/ml

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