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Interleukin-4 (IL-4) ELISA Kit

INTENDED USE

Human IL-4 ELISA kit is designed for measuring in vitro quantitative concentrations of interleukin-4 (IL-4) present in human tissue homogenates, plasma (EDTA, heparin, citrate), biological fluids and serum samples. This assay has a minimum detection limit of 18.75 pg/ml.

BACKGROUND

Interleukin-4 (IL-4) was first characterised as B cell stimulatory factor (BSF-1) and B-cell differentiation factor (BCDFg). It has been subsequently revealed that IL-4 is a pleiotropic cytokine with many immune response modulating functions on a large number of cell types which include fibroblasts, osteoblasts, endothelial cells, hepatocytes, keratinocytes, astrocytes, mast cells, macrophages and T cells. It is essentially an immunomodulatory cytokine that has the ability to inhibit the growth of tumour cells. Interleukin-4 is known to be produced by mast cells, fetal thymocytes, CD8+ T cells, basophils, CD4+ TH0 and TH2 cells.

The human cDNA is made up of a single open reading frame which encodes a protein of 153 amino acids and this also includes a putative signal peptide. The human IL-4 gene is located on chromosome 5 and it occurs as a single copy in the haploid genome. There are structural and functional similarities between human and mouse IL-4 genes and their proteins, both human and mouse proteins have multiple potential glycosylation sites and six cysteines which are linked by intro-molecular disulfide bridges.

There are evidence that IL-4 can act as an autocrine growth factor in pancreatic cancer cells, this has been found to give rise to the possibility that cancer-derived IL-4 being able to suppress the cancer-directed immune-surveillance in vivo, in addition to its growth-promoting effects. This process can result in the facilitation of both pancreatic tumor growth and metastasis.

IL-4 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-4 antibody.
  • Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant human interleukin-4.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-4 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-4 (IL-4) using this human IL-4 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-4 (IL-4): ELISA
– Specificity: Highly specific for IL-4, no cross reactivity or interference between IL-4 and analogues was detected.
– Recovery: Serum (86 – 105%), EDTA Plasma (85 – 105%), Heparin Plasma (86 – 99%).
– Linearity: Serum (85 – 105%), EDTA Plasma (83 – 101%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Control of IL-4 expression in T helper 1 and 2 cells. Immunology. (2008) 124 (4): 437-44. Review. Gilmour J. and Lavender P.
  2. IL-4 in the brain: a cytokine to remember. J Immunol. (2012) 189 (9): 4213-9. Review. Gadani S.P., et al.
  3. Spatial regulation of IL-4 signalling in vivo. Cytokine. (2015) 75 (1): 51-6. Review. Redpath S.A., et al.
  4. Anti-interleukin-4 therapy. Immunol Allergy Clin North Am. (2004) 24 (4): 599-614. Review. Steinke J.W.
  5. History of interleukin-4. Cytokine. (2015) 75 (1): 3-7. Review. Paul W.E.
  6. Gene therapy and biologic therapy with interleukin-4. Curr Gene Ther. (2002) 2 (4): 437-50. Review. Okada H. and Kuwashima N.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-4 (IL-4) ELISA Kit
  • Reactivity: Human
  • Sample Type: Biological Fluids, Plasma (Heparin, EDTA, Citrate), Serum, Tissue Homogenates
  • Sensitivity: 18.75 pg/ml

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