GET A QUOTE

Interleukin-10 (IL-10) ELISA Kit

INTENDED USE

Human IL-10 ELISA kit can be used for determining in vitro quantitative amounts of interleukin-10 (IL-10, interleukin 10) in human serum, biological fluids, plasma and many other biological fluids. This assay has a minimum detection limit of 4.688 pg/ml.

BACKGROUND

Interleukin-10 (IL-10) is a member of the four a-helix bundle family with a molecular mass of 39 kDa (160 amino acids) and in solution human IL-10 is only functional has a homodimer. It is essentially a pleiotropic cytokine which plays important roles in regulating lymphoid and myeloid cell functions. IL-10 is a potent suppressor of the effector functions of T-cells, macrophages and NK cells since it has the ability to block the synthesis of cytokines and several accessory cell functions of macrophages. Interleukin-10 is found to be expressed by a number of different cells which includes B cells derived from peripheral blood, EBV-transformed B cell lines, Burkitt’s lymphoma, placental trophoblasts, monocytes, bronchial epithelial cells, AIDS lymphomas, certain melanomas and carcinomas.

Human IL-10 gene is found to be located on chromosome 1 and it is often occurring as a single copy within the genome. It displays strong amino acid sequence and DNA sequence homology to the murine IL-10, it also exhibits similarity to the open reading frame present in the Epstein Barr virus genome and BCRF1. This led to implications there is a possibility that IL-10 may also play a role in the host-virus interactions.

The immunosuppressive properties that are shown by IL-10 indicate a potential clinical use for IL-10 in suppressing rejections of grafts following organ transplantations. There is a further possibility of it exerting a strong anti-inflammatory activities too. In many diseases for example Leishmania, Schistosoma mansoni, Trypanosoma and Toxoplasma gondii infections the levels of interleukin-10 have been found to be elevated. High concentrations of IL-10 are also found in mycobacterial infections (Mycobacterium leprae, Mycobacterium avium and Mycobacterium tuberculosis) and in retroviral infections inducing immunodeficiency.

IL-10 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-10 antibody.
  • Standards: Concentrations 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant human interleukin-10.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-10 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-10 (IL-10) using this human IL-10 ELISA kit was approximately 4.688 pg/ml. The dynamic assay range for this kit is 7.813 – 500 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-10 (IL-10): ELISA
– Specificity: Highly specific for IL-10, no cross reactivity or interference between IL-10 and analogues was detected.
– Recovery: Serum (86 – 99%), EDTA Plasma (85 – 103%), Heparin Plasma (86 – 95%).
– Linearity: Serum (89 – 101%), EDTA Plasma (83 – 98%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Interleukin 10 in the tumor microenvironment: a target for anticancer immunotherapy. Immunol Res. (2011) 51 (2-3): 170-82. Review. Sato T., et al.
  2. Current status of interleukin-10 and regulatory T-cells in cancer. Curr Opin Oncol. (2013) 25 (6): 637-45. Review. Dennis K.L., et al.
  3. Interleukin-10 protects against atherosclerosis by modulating multiple atherogenic macrophage function. Thromb Haemost. (2015) 113 (3): 505-12. Review. Han X. and Boisvert W.A.
  4. Interleukin-10-producing B cells and the regulation of immunity. Curr Top Microbiol Immunol. (2014) 380: 69-92. Review. Hilgenberg E., et al.
  5. Targeting IL-10 in auto-immune diseases. Cell Biochem Biophys. (2014) 70 (1): 37-49. Tian G., et al.
  6. The therapeutic potential of interleukin-10 in neuroimmune diseases. Neuropharmacology. (2015) 96 (Pt A): 55-69. Review. Kwilasz A.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-10 (IL-10) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum, Biological Fluids, Tissue Homogenates
  • Sensitivity: 4.688 pg/ml

OTHER RELATED ELISA KITS

 

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford