Interleukin-10 (IL-10) ELISA Kit

INTENDED USE

Human IL-10 ELISA kit can be used for determining in vitro quantitative amounts of interleukin-10 (IL-10, interleukin 10) in human serum, biological fluids, plasma and many other biological fluids. This assay has a minimum detection limit of 4.688 pg/ml.

BACKGROUND

Interleukin-10 (IL-10) is a member of the four a-helix bundle family with a molecular mass of 39 kDa (160 amino acids) and in solution human IL-10 is only functional has a homodimer. It is essentially a pleiotropic cytokine which plays important roles in regulating lymphoid and myeloid cell functions. IL-10 is a potent suppressor of the effector functions of T-cells, macrophages and NK cells since it has the ability to block the synthesis of cytokines and several accessory cell functions of macrophages. Interleukin-10 is found to be expressed by a number of different cells which includes B cells derived from peripheral blood, EBV-transformed B cell lines, Burkitt’s lymphoma, placental trophoblasts, monocytes, bronchial epithelial cells, AIDS lymphomas, certain melanomas and carcinomas.

Human IL-10 gene is found to be located on chromosome 1 and it is often occurring as a single copy within the genome. It displays strong amino acid sequence and DNA sequence homology to the murine IL-10, it also exhibits similarity to the open reading frame present in the Epstein Barr virus genome and BCRF1. This led to implications there is a possibility that IL-10 may also play a role in the host-virus interactions.

The immunosuppressive properties that are shown by IL-10 indicate a potential clinical use for IL-10 in suppressing rejections of grafts following organ transplantations. There is a further possibility of it exerting a strong anti-inflammatory activities too. In many diseases for example Leishmania, Schistosoma mansoni, Trypanosoma and Toxoplasma gondii infections the levels of interleukin-10 have been found to be elevated. High concentrations of IL-10 are also found in mycobacterial infections (Mycobacterium leprae, Mycobacterium avium and Mycobacterium tuberculosis) and in retroviral infections inducing immunodeficiency.

IL-10 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human IL-10 antibody.
• Standards: Concentrations 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant human interleukin-10.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled IL-10 Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-10 (IL-10) using this human IL-10 ELISA kit was approximately 4.688 pg/ml. The dynamic assay range for this kit is 7.813 – 500 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-10 (IL-10): ELISA
– Specificity: Highly specific for IL-10, no cross reactivity or interference between IL-10 and analogues was detected.
– Recovery: Serum (86 – 99%), EDTA Plasma (85 – 103%), Heparin Plasma (86 – 95%).
– Linearity: Serum (89 – 101%), EDTA Plasma (83 – 98%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Interleukin 10 in the tumor microenvironment: a target for anticancer immunotherapy. Immunol Res. (2011) 51 (2-3): 170-82. Review. Sato T., et al.
2. Current status of interleukin-10 and regulatory T-cells in cancer. Curr Opin Oncol. (2013) 25 (6): 637-45. Review. Dennis K.L., et al.
3. Interleukin-10 protects against atherosclerosis by modulating multiple atherogenic macrophage function. Thromb Haemost. (2015) 113 (3): 505-12. Review. Han X. and Boisvert W.A.
4. Interleukin-10-producing B cells and the regulation of immunity. Curr Top Microbiol Immunol. (2014) 380: 69-92. Review. Hilgenberg E., et al.
5. Targeting IL-10 in auto-immune diseases. Cell Biochem Biophys. (2014) 70 (1): 37-49. Tian G., et al.
6. The therapeutic potential of interleukin-10 in neuroimmune diseases. Neuropharmacology. (2015) 96 (Pt A): 55-69. Review. Kwilasz A.J., et al.

ADDITIONAL INFORMATION

• Full Name: Interleukin-10 (IL-10) ELISA Kit
• Reactivity: Human
• Sample Type: Plasma, Serum, Biological Fluids, Tissue Homogenates
• Sensitivity: 4.688 pg/ml

OTHER RELATED ELISA KITS

• IL-16 ELISA Kit
• IL-11 ELISA Kit
• IL-3 ELISA Kit