IL-10 ELISA Kit (Interleukin-10)
INTENDED USE
Human IL-10 ELISA kit can be used for determining in vitro quantitative amounts of interleukin-10 (IL-10, interleukin 10) in human serum, biological fluids, plasma and many other biological fluids. This assay has a minimum detection limit of 4.688 pg/ml.
BACKGROUND
Interleukin-10 (IL-10) is a four a-helix bundle family with a molecular mass of 39 kDa, in solution it is only functional has a homodimer. It is essentially a pleiotropic cytokine that has important roles in regulating lymphoid and myeloid cell functions. It is a potent suppressor of the effector functions of T-cells, macrophages and NK cells since it has the ability to block the synthesis of cytokines and several accessory cell functions of macrophages.
IL-10 is expressed by a number of different cells which includes B cells derived from peripheral blood, EBV-transformed B cell lines, Burkitt’s lymphoma, placental trophoblasts, monocytes, bronchial epithelial cells, AIDS lymphomas, certain melanomas and carcinomas. It displays strong amino acid sequence and DNA sequence homology to the murine, it also exhibits similarity to the open reading frame present in the Epstein Barr virus genome and BCRF1. This led to implications there is a possibility that it may also play a role in the host-virus interactions.
The immunosuppressive properties that are shown to indicate a potential clinical use in suppressing rejections of grafts following organ transplantations. There is a further possibility of it exerting a strong anti-inflammatory activities too.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human IL-10 antibody.
- Standards: Concentrations 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-Labelled-Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
SENSITIVITY
The minimum detection sensitivity level of interleukin-10 (IL-10) using this IL-10 ELISA kit was approximately 4.688 pg/ml. The dynamic assay range for this kit is 7.813 – 500 pg/ml.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for IL-10, no cross reactivity or interference between IL-10 and analogues was detected.
– Recovery: Serum (86 – 99%), EDTA Plasma (85 – 103%), Heparin Plasma (86 – 95%).
– Linearity: Serum (89 – 101%), EDTA Plasma (83 – 98%), Heparin Plasma (80 – 93%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
REFERENCES
- Interleukin 10 in the tumor microenvironment: a target for anticancer immunotherapy. Immunol Res. (2011) 51 (2-3): 170-82. Review. Sato T., et al.
- Current status of interleukin-10 and regulatory T-cells in cancer. Curr Opin Oncol. (2013) 25 (6): 637-45. Review. Dennis K.L., et al.
- Interleukin-10-producing B cells and the regulation of immunity. Curr Top Microbiol Immunol. (2014) 380: 69-92. Review. Hilgenberg E., et al.
- Targeting IL-10 in auto-immune diseases. Cell Biochem Biophys. (2014) 70 (1): 37-49. Tian G., et al.
ADDITIONAL INFORMATION
- Full Name: IL-10 ELISA Kit (Interleukin-10)
- Reactivity: Human
- Sample Type: Plasma, Serum, Biological Fluids, Tissue Homogenates
- Sensitivity: 4.688 pg/ml
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