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Interleukin-2 (IL-2) ELISA Kit

INTENDED USE

Interleukin-2 ELISA kit is a method for determining in vitro quantitative amounts of interleukin-2 (IL-2) in human serum, cell culture supernatant, plasma (heparin, citrate, EDTA). This assay has a minimum detection limit of < 0.1 pg/ml.

BACKGROUND

Interleukin-2 (IL-2) which was also originally known as T-cell growth factor, is produced by antigen- or lectin-activated T cells and is a powerfully immune-regulatory lymphokine. It is not only formed by the stimulation of mature T lymphocytes but also constitutively by a number of T-cell lymphoma cell lines. It has vital functions in the regulation of activation and proliferation of lymphocytes which have already been primed by antigens.

Interleukin-2 also has a central role in the expansion of B-cells, T-cells and natural killer cells during certain phases of their response. The human gene IL 2 is located on chromosome 4. Human IL-2 is made up of 153 amino acids in the precursor form or 133 amino acids in the mature form and it is a 15.5KDa glycoprotein. Due to its central role in the immune response process, IL-2 has become a very important for many therapeutic and diagnostic implications.

There is evidence to suggest that it may also display anti-tumoral effects which can be particularly useful in cancer therapy. By monitoring the levels of interleukin 2 in serum this can provide a better insight into several pathological situations such as rheumatoid arthritis, transplant rejection, cancer, multiple sclerosis, type I diabetes, systemic lupus erythematosus and infectious diseases. However, this issue with IL-2 toxicity is still a major concern and merit investigation.

IL-2 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-2 antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant human interleukin-2.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-2 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of IL-2 (interleukin-2) using current human IL-2 ELISA kit was approximately 9.375 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-2 (IL-2): ELISA
– Specificity: Highly specific for IL-2, no cross reactivity or interference between IL-2 and analogues was detected.
– Recovery: Serum (87 – 105%), EDTA Plasma (85 – 105%), Heparin Plasma (90 – 105%).
– Linearity: Serum (85 – 105%), EDTA Plasma (82 – 98%), Heparin Plasma (95 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Central role for interleukin-2 in type 1 diabetes. Diabetes. (2012) 61 (1): 14-22. Review. Hulme M.A., et al.
  2. Inhibitors of interleukin-2 inducible T-cell kinase as potential therapeutic candidates for the treatment of various inflammatory disease conditions. Eur J Pharm Sci. (2012) 47 (3): 574-88. Review. Kaur M., et al.
  3. IL-2: the first effective immunotherapy for human cancer. J Immunol. (2014) 192 (12): 5451-8. Review. Rosenberg S.A.
  4. Interleukin 2 in cancer therapy. Curr Med Chem. (2010) 17 (29): 3297-302. Review. Antony G.K. and Dudek A.Z.
  5. The regulatory, inflammatory, and T cell programming roles of interleukin-2 (IL-2). J Autoimmun. (2008) 31 (1): 7-12. Review. Lan R.Y., et al.
  6. The biology of interleukin-2. Annu Rev Immunol. (2008) 26: 453-79. Review. Malek T.R.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-2 (IL-2) ELISA Kit
  • Reactivity: Human
  • Sample Type: Tissue Homogenates, Serum, Biological Fluids, Plasma (Citrate, EDTA, Heparin)
  • Sensitivity: 9.375 pg/ml

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