IL-1 Alpha ELISA Kit (Interleukin-1 Alpha)
INTENDED USE
Human IL-1 alpha ELISA kit is a method for measuring in vitro quantitative levels of interleukin-1 alpha (IL-1α, IL-1a, IL-1 alpha) in human biological fluids, serum, plasma (EDTA, citrate, heparin) and tissue homogenates. This assay has a minimum detection limit of 2.344 pg/ml.
BACKGROUND
Interleukin-1 alpha (IL-1 alpha, IL-1α or IL-1a) represents biologically active cell-derived mono nuclear proteins that are involved in immune responses and inflammatory reactions. There are two distinct proteins which have be discovered namely IL-1 alpha and IL-1 beta. They are both biologically distinct but distantly related, sharing similarities such as molecular weight, same receptors on target cells and similar biological effects. Also, they are both polypeptidic cytokines that play vital functions in immunologic reactions, tissue repair and inflammation. IL-1α is however predominantly a cell-associated molecule when compared to IL-1beta which is a secreted molecule.
IL-1 alpha is usually synthesized primarily as a 31 kDa precursor which lacks a signal peptide. This precursor is cleaved through the cysteine protease calpain, leading to the production of 17.5 kDa mature IL-1 molecule. It can also induce the production of: GM-CSF, IL-4 from activated T cells, IL-2 receptors and IL-2. IL-1α can increase immunoglobulin synthesis, stimulate B cell proliferation and maturation, induce prostaglandin synthesis in endothelial cells and smooth muscle cells, NK cell activation and LAK production associated with other cytokines, cartilage and calcium resorption in bones and collagenase production in synovial cells.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human IL-1 alpha antibody.
- Standards: Concentrations 0, 3.906, 7.812, 15.625, 31.25, 62.5, 125, 250 pg/ml, made up from lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-Labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
SENSITIVITY
The minimum detection sensitivity level of interleukin-1 alpha (IL-1 alpha, IL-1α, IL-1a) using current IL-1 alpha ELISA kit was approximately 2.344 pg/ml. The dynamic range for this assay is 3.906 – 250 pg/ml.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for IL-1 alpha, no cross reactivity or interference between IL-1 alpha and analogues was detected.
– Recovery: Serum (97 – 102%), EDTA Plasma (86 – 103%), Heparin Plasma (86 – 103%).
– Linearity: Serum (89 – 102%), EDTA Plasma (88 – 99%), Heparin Plasma (82 – 91%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
REFERENCES
- Interleukin 1α and the inflammatory process. Nat Immunol. (2016) 17 (8): 906-13. Review. Di Paolo N.C. and Shayakhmetov D.M.
- Molecular cloning, overexpression and characterization of human interleukin 1alpha. Biochem Biophys Res Commun. (2007) 360 (3): 604-8. Rajalingam D., et al.
- Gene polymorphism of interleukin-1 alpha and beta in keratocystic odontogenic tumors. J Oral Pathol Med. (2012) 41 (9): 697-701. Eshghyar N., et al.
- Interleukin-1α and brain inflammation. IUBMB Life. (2015) 67 (5): 323-30. Review. Brough D. and Denes A.
ADDITIONAL INFORMATION
- Full Name: IL-1 Alpha ELISA Kit (Interleukin-1 Alpha)
- Reactivity: Human
- Sample Type: Tissue Homogenates, Serum, Plasma, Biological Fluid
- Sensitivity: 2.344 pg/ml
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