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Interleukin-15 (IL-15) ELISA Kit

INTENDED USE

Human IL-15 ELISA kit is intended for measuring in vitro quantitative concentrations of interleukin-15 (IL-15) in human tissue homogenates, plasma (EDTA, heparin), biological fluids and serum. This assay has a minimum detection limit of 9.375 pg/ml.

BACKGROUND

Interleukin-15 (IL-15), at maturity is able to reach 114 amino acid residues and is a modest 14-15 kDa glycoprotein that can be produced by dendritic and monocyte cells. IL-15 belongs to the four a helix bound cytokine family, other members include IL-2, IL-3, IL-5, IL-6, IL-7 and IL-9. At present the structure of IL-15 has not been determined, however, it has been predicted to be similar to IL-2 and other members of this family. The mRNA of IL-15 has been identified in monocytes, dendritic cells, epithelial cells, fibroblasts, placenta, kidneys, heart, skeletal muscle and lungs.

Human IL-15 is known to display approximately 97% and 73% amino acid sequence identity with simian and mouse IL-15, respectively. Both it is found that human and simian IL-15 molecules are active with mouse cells. Abnormal levels of IL-15 expression have been discovered in inflammatory bowel disease, rheumatoid arthritis and many diseases that have been associated with the retroviruses HIV and HTLV-I. The biological activities of IL-15 are very comparable to IL-2 such as IL-15 being able to induce the proliferation of activated CD4+, CD8+, CD4+CD8+, CD4-CD8- and dendritic epidermal T cells. The activation, development and survival of natural killer (NK) cells has been associated to IL-15.

Interleukin-15 also affects the non-lymphoid tissues, such as brain, muscle, mast cells and microglia. In combination with recombinant CD40L, IL-15 can be a potent inducer of polyclonal IgA, IgG1 and IgM secretion, but it is unable to induce the production of IgE or IgG4. The use of anti-IL-15 therapy with the combination of classic immunosuppression therapy could be beneficial in the prevention or treatment of acute and especially chronic, allograft rejection.

IL-15 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human IL-15 antibody.
  • Standards: Concentrations 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml, made up from lyophilized recombinant human interleukin-15.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled IL-15 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-15 (IL-15) using this human IL-15 ELISA kit was approximately 9.375 pg/ml. The dynamic range for this assay is 15.625 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Interleukin-15 (IL-15): ELISA
– Specificity: Highly specific for IL-15, no cross reactivity or interference between IL-15 and analogues was detected.
– Recovery: Serum (85 – 103%), EDTA Plasma (85 – 102%), Heparin Plasma (89 – 103%).
– Linearity: Serum (86 – 90%), EDTA Plasma (82 – 99%), Heparin Plasma (82 – 95%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. IL15 and T-cell Stemness in T-cell-Based Cancer Immunotherapy. Cancer Res. (2015) 75 (24): 5187-93. Review. Pilipow K., et al.
  2. Interleukin-15: new kid on the block for antitumor combination therapy. Cytokine Growth Factor Rev. (2015) 26 (1): 15-24. Van den Bergh J.M., et al.
  3. IL-15 functions as a danger signal to regulate tissue-resident T cells and tissue destruction. Nat Rev Immunol. (2015) 15 (12): 771-83. Review. Jabri B. and Abadie V.
  4. Interleukin-15 in the treatment of cancer. Expert Rev Clin Immunol. (2014) 10 (12): 1689-701. Review. Waldmann T.A.
  5. The role of inflammatory and anti-inflammatory cytokines in the pathogenesis of osteoarthritis. Mediators Inflamm. (2014) 2014: 561459. Review. Wojdasiewicz P., et al.
  6. Therapeutic potential of IL-15 in rheumatoid arthritis. Hum Immunol. (2015) 76 (11): 812-8. Review. Yang X.K., et al.

ADDITIONAL INFORMATION

  • Full Name: Interleukin-15 (IL-15) ELISA Kit
  • Reactivity: Human
  • Sample Type: Biological Fluids, Plasma (Heparin, EDTA), Serum, Tissue Homogenates
  • Sensitivity: 9.375 pg/ml

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