GET A QUOTE

IL-7 ELISA Kit (Interleukin-7)

INTENDED USE

Human IL-7 ELISA kit is a procedure of detecting in vitro quantitative amounts of interleukin-7 (IL-7) present in human serum, tissue homogenates, other biological fluids and plasma. This assay has a minimum detection limit of 4.688 pg/ml.

BACKGROUND

Interleukin-7 (IL-7) is a 17.4 kDa protein that consists of six exons, it is also produced by various cells including; endothelial cells, dendritic cells, keratinocytes, hepatocytes and neurons. It can affect survival and proliferation of mature T cells, enhances proliferation and survival of T cell precursors (human and mice) and B cell precursors (mice only), potent enhancer of T cell function and IFN-γ production.

IL-7 is a critical cytokine for both normal T and B lymphopoiesis, a mobilizer of pluripotent stem cells and myeloid progenitors. Possessing vital roles in enhancing T cell function and also able to induces cytokine expression in monocytes. Preclinical studies have indicated that it is capable of accelerating murine lymphocyte regeneration following chemotherapy or bone marrow transplantation treatment, inducing anti-tumor effects (mice only) and useful in expanding anti-HIV-specific human T cells.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti IL-7 antibody.
  • Standards: Concentrations 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-Labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of interleukin-7 (IL-7) using this IL-7 ELISA kit was approximately 4.688 pg/ml. The dynamic range for this assay is 7.813 – 500 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-7, no cross reactivity or interference between IL-7 and analogues was detected.
– Recovery: Serum (86 – 103%), EDTA Plasma (89 – 98%), Heparin Plasma (89 – 98%).
– Linearity: Serum (85 – 104%), EDTA Plasma (83 – 101%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Interleukin 7 and thymic stromal lymphopoietin: from immunity to leukemia. Cell Mol Life Sci. (2014) 71 (3): 365-78. Review. Tal N., et al.
  2. Interleukin-7 and immune reconstitution in cancer patients: a new paradigm for dramatically increasing overall survival. Target Oncol. (2012) 7 (1): 55-68. Review. Morre M. and Beq S.
  3. Mechanism of Action of IL-7 and Its Potential Applications and Limitations in Cancer Immunotherapy. Int J Mol Sci. (2015) 16 (5): 10267-80. Review. Gao J., et al.
  4. Interleukin-7-receptor alpha as a potential therapeutic target in transplantation. Arch Immunol Ther Exp (Warsz). (2009) 57 (4): 253-61. Review. Racapé M., et al.

ADDITIONAL INFORMATION

  • Full Name: IL-7 ELISA Kit (Interleukin-7)
  • Reactivity: Human
  • Sample Type: Serum, Biological Fluids, Tissue Homogenates, Plasma
  • Sensitivity: 4.688 pg/ml

OTHER RELATED ELISA KITS

Online Enquiry Form

"*" indicates required fields

Please check mark information required:

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford