Human IL-11 ELISA kit is intended for analysing in vitro quantitative levels of interleukin-11 (IL-11, interleukin 11) present in human serum, biological fluids, tissue homogenates and plasma. This assay has a minimum detection limit of 18.75 pg/ml.
Interleukin-11 (interleukin 11, IL-11) can also be called adipogenesis inhibitory factor (AGIF) or oprelvekin and it belongs to a family of cytokines that are known as GP130. There are many cell and tissue which can produce IL-11 throughout the body, for example lung, connective tissues, thymus, CNS, bone, skin, testis and uterus. One of the main functions of IL-11 is to promote both in vitro and in vivo primary and secondary immune responses which can be responsible for modulating the antigen-specific antibody reactions. Interleukin-11 has the ability to exert its biological activity via IL-11 receptor by utilising the gp130 molecule as the signalling component of its receptor.
IL-11 is a highly conserved precursor protein which has a total molecular mass of 19kDa and is composed of 199 amino acids. The initial 21 amino acids are referred to as the typical leader sequence for secreted proteins. This first 21 amino acid sequence is removed when the 178 amino acid is being released from the cell. The interleukin-11 molecule is thermally stable and also does not contain any cysteine residues.
Interleukin-11 can be found to display activity in bone metabolism and during the protection or restoration of the gastrointestinal mucosa. Also, human endometrial stromal cells can form biologically active IL-11 which can be responsible for promoting progesterone-induced decidualization. There are a number of reports that indicate abnormal IL-11 concentrations in serum or plasma being related to urogenital infection, rheumatoid arthritis and immune thrombocytopenia. IL-11 has also been linked to aseptic loosening of total hip replacement implants.
IL-11 ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-human IL-11 antibody.
- Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml, made up from lyophilized recombinant human interleukin-11.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled IL-11 Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
The minimum detection sensitivity level of IL-11 (interleukin-11 and interleukin 11) using current human IL-11 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2,000 pg/ml.
– Interleukin-11 (IL-11): ELISA
– Specificity: Highly specific for IL-11, no cross reactivity or interference between IL-11 and analogues was detected.
– Recovery: Serum (89 – 103%), EDTA Plasma (93 – 104%), Heparin Plasma (89 – 101%).
– Linearity: Serum (85 – 102%), EDTA Plasma (87 – 96%), Heparin Plasma (84 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.
- The role of IL-11 in immunity and cancer. Cancer Lett. (2016) 373 (2): 156-63. Review. Xu D.H., et al.
- Interleukin-11: A Multifunctional Cytokine with Intrinsically Disordered Regions. Cell Biochem Biophys. (2016) 74 (3): 285-96. Review. Permyakov E.A., et al.
- Cytokine signalling via gp130 in gastric cancer. Biochim Biophys Acta. (2009) 1793 (11): 1623-33. Review. Howlett M., et al.
- IL-11 signaling as a therapeutic target for cancer. Immunotherapy. (2015) 7 (4): 441-53. Review. Putoczki T.L. and Ernst M.
- Interleukin-11. A gp130 cytokine. Ann N Y Acad Sci. (1998) 856: 12-21. Review. Trepicchio W.L. and Dorner A.J.
- The biology of interleukin 11. Stem Cells. (1993) 11 Suppl 2: 156-62. Review. Neben S. and Turner K.
- Full Name: Interleukin-11 (IL-11) ELISA Kit
- Reactivity: Human
- Sample Type: Biological Fluids, Serum, Tissue Homogenates, Plasma
- Sensitivity: 18.75 pg/ml