ICAM-2 ELISA Kit (Intercellular Adhesion Molecule-2)


Human ICAM-2 ELISA kit is designed for determining in vitro quantitative concentrations of intercellular adhesion molecule-2 (ICAM2, CD102, ICAM-2) in human serum, plasma, cell culture supernatant, other biological fluids and cell lysates. This assay has a minimum sensitivity limit of 0.188 ng/ml.


Intercellular adhesion molecule-2 (ICAM-2, ICAM2) which can also be referred to as CD102, is a 55 kD type I transmembrane glycoprotein that contains approx. 2-9 immunoglobulin-like C2-type domains and has the ability to bind leukocyte adhesion LFA-1 protein. It is also found to regulate spermatid adhesion on Sertori cells that are located on the apical side of the blood-testis barrier.

ICAM-2 is known to be constitutively expressed on mainly vascular endothelial cells and some monocytes, lymphocytes and platelets. It is a counter receptor of MAC-1, LFA-1, CD11a/CD18 and DC-SIGN, where it is thought to be responsible for lymphocyte re-circulation and in the co-stimulation of immune response. It essentially can mediate adhesive interactions which are vital for antigen-specific lymphocyte recirculation, NK-cell mediated clearance, immune response and surveillance.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti ICAM-2 antibody.
  • Standards: Concentrations 0, 3.12, 0.625, 1.25, 2.5, 5.0, 10.0, 20.0 ng/ml, made up from lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-Labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


The minimum detection sensitivity level of intercellular adhesion molecule-2 (ICAM-2, CD102, ICAM2) using current human ICAM-2 ELISA kit was 0.188 ng/ml. The dynamic range for this assay is 0.313 – 20 ng/ml.


– Specificity: Highly specific for ICAM-2, no cross reactivity or interference between ICAM-2 and analogues was detected.
– Recovery: Serum (85 – 102%), EDTA Plasma (91 – 104%), Heparin Plasma (88 – 103%).
– Linearity: Serum (88 – 105%), EDTA Plasma (84 – 101%), Heparin Plasma (81 – 94%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.


  1. Crystal structure of ICAM-2 reveals a distinctive integrin recognition surface. Nature. (1997) 387 (6630): 312-5. Casasnovas J.M., et al.
  2. Adhesion molecules in lymphoma metastasis. Cancer Metastasis Rev. (1991) 10 (1): 33-48. Review. Roos E.
  3. Platelet associated fibrinogen and ICAM-2 induce firm adhesion of neutrophils under flow conditions. Thromb Haemost. (1998) 80 (3): 443-8. Kuijper P.H., et al.
  4. Leukocyte-endothelial cell adhesive receptors. Clin Exp Rheumatol. (1994) 12 Suppl 10: S25-8. Review. Dejana E., et al.


  • Full Name: ICAM-2 ELISA Kit (Intercellular Adhesion Molecule-2)
  • Reactivity: Human
  • Sample Type:
  • Serum, Tissue Homogenates, Plasma, Other Biological Fluids
  • Sensitivity: 0.188 ng/ml


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