IGFBP-1 ELISA Kit (Insulin Growth Factor Binding Protein 1)


Human IGFBP-1 ELISA kit is a reliable quantitative procedure for measuring insulin like growth factor 1 (IGFBP1, IGFBP-1) in human serum, plasma, body fluids, tissue lysates and cell culture supernatant. This assay has a minimum sensitivity detection limit of 0.02 ng/ml.


Insulin like growth factor binding protein 1 (IGFBP-1 or IBP-1) is also called by many other names which include placental protein-12, growth hormone independent binding protein, binding protein-25, 26 and 28, amniotic fluid binding protein(AFBP) and alpha-pregnancy-associated endometrial globulin. It is a low molecular weight protein that is synthesised by the liver, secretory endometrium or decidua.

IGFBP-1 has the ability to bind with high affinity to IGF 1 and IGF 2, binding of this protein can prolong the half-life as well as altering their interaction with cell surface receptors. It is described as a paracrine regulator for cell growth. IGF binding proteins are known to expressed at a number of different concentrations, in many cells and have vital roles in regulating the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb, it is a single copy gene that is located on chromosome 7.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with anti-human IGFBP-1-antibody. Wells are separately breakable.
  • Standards A-G: Lyophilized, concentrations are 0, 01, 0.5, 1, 2, 4, 8 ng/mL.
  • Control Serum 1 And Control Serum 2: Lyophilised, human serum.
  • Antibody Conjugate: biotinylated anti-hIGFBP-1 antibody.
  • Enzyme Conjugate: HRP (Horseradish-Peroxidase)-labelled Streptavidin.
  • Washing Buffer (20x Concentrated).
  • Substrate: HRP substrate, stabilised tetramethylbencidine.
  • Stopping Solution: 0.2 M sulphuric acid.
  • Sealing Tape: Covering the microtiter plate.


The minimum sensitivity detection limit of human insulin like growth factor 1 (IGFBP-1, IGFBP1) using current IGFBP-1 ELISA kit was approximately 0.02 ng/ml. The dynamic range for this assay is 0 – 8 ng/ml.


– Insulin Like Growth Factor 1 (IGFBP-1, IGFBP1): ELISA
– Analytical Sensitivity: 0.02 ng/ml
– Linearity Of Dilution: 5.53 – 7.44%
– Cross Reactivity: Highly specific, does not cross react with recombinant IGFBP-2 or IFGBP-3.
– Intra Assay Variation: 5.23 – 7.36%
– Inter Assay Variation: 5.87 – 6.81%


  1. Insulin like growth factor binding protein 1: recent findings and new directions. Proc Soc Exp Biol Med. (1997) 216 (3): 319-57. Review. Lee P.D., et al.
  2. Organization of the gene encoding the insulin-like growth factor binding protein IBP-1. Biochem. Biophys. Res. Commun. (1988) 157: 898-907. Brinkman, A., et al.
  3. Multifaceted roles for IGFBP-1 in human endometrium during implantation and pregnancy. Ann N Y Acad Sci. (1997) 828: 146-56. Review. Giudice LC.
  4. Regulation of IGFBP-1 in humans. Prog Growth Factor Res. (1995) 6 (2-4): 449-56. Review. Brismar K., Hilding A. and Lindgren B.


  • Full Name: IGFBP-1 ELISA Kit (Insulin Growth Factor Binding Protein 1)
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant, Body Fluids
  • Sensitivity: 0.02 ng/ml


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford